MACROD2 sirna

MACROD2 siRNA (Mouse)

Cat. Num. AAA8226151

• Gene Names: Macrod2; 1110033L15Rik; 2610107G07Rik; 2900006F19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MACROD2; MACROD2 siRNA (Mouse); O-acetyl-ADP-ribose deacetylase MACROD2; MACRO domain-containing protein 2; [Protein ADP-ribosylglutamate] hydrolase; MACROD2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MACROD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 475

• Applicable Applications for MACROD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MACROD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MACROD2 sirna

siRNA to inhibit MACROD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148227060
• NCBI GeneID: 72899
• NCBI Accession #: NP_001013824.2
• NCBI GenBank Nucleotide #: NM_001013802.3
• UniProt Accession #: Q3UYG8
• Molecular Weight: 52,145 Da
• NCBI Official Full Name: O-acetyl-ADP-ribose deacetylase MACROD2 isoform 1
• NCBI Official Synonym Full Names: MACRO domain containing 2
• NCBI Official Symbol: Macrod2
• NCBI Official Synonym Symbols: 1110033L15Rik; 2610107G07Rik; 2900006F19Rik
• NCBI Protein Information: O-acetyl-ADP-ribose deacetylase MACROD2
• UniProt Protein Name: O-acetyl-ADP-ribose deacetylase MACROD2
• Protein Family: O-acetyl-ADP-ribose deacetylase
• UniProt Gene Name: Macrod2
• UniProt Entry Name: MACD2_MOUSE

Uniprot Description

MACROD2: Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-

Cellular Component: nucleus

Molecular Function: (3,5-dichlorophenylurea)acetate amidohydrolase activity; single-strand selective uracil DNA N-glycosylase activity; deacetylase activity; uracil DNA N-glycosylase activity; hydrolase activity; DNA N-glycosylase activity; hydrolase activity, acting on glycosyl bonds; purine-specific mismatch base pair DNA N-glycosylase activity; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; DNA-3-methyladenine glycosylase III activity; iprodione amidohydrolase activity; indoleacetamide hydrolase activity; protein N-terminal asparagine amidohydrolase activity; histone deacetylase activity; O-succinylbenzoate synthase activity

Biological Process: purine nucleoside metabolic process; protein amino acid de-ADP-ribosylation; response to DNA damage stimulus