LPCAT2 sirna

LPCAT2 siRNA (Mouse)

Cat. Num. AAA8214935

• Gene Names: Lpcat2; Aytl1; Aytl1a; lpafat1; A330042H22
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LPCAT2; LPCAT2 siRNA (Mouse); AYTL1; AYTL1A; LPCAT2A; Lysophosphatidylcholine acyltransferase 2; LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-AGP acyltransferase 11; 1-AGPAT 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 1; LPCAT2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LPCAT2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 544

• Applicable Applications for LPCAT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LPCAT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LPCAT2 sirna

siRNA to inhibit LPCAT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27370522
• NCBI GeneID: 270084
• NCBI Accession #: NP_766602.1
• NCBI GenBank Nucleotide #: NM_173014.1
• UniProt Accession #: Q8BYI6; A3KBM0; A9Q1G2; A9Q1G3
• Molecular Weight: 25,486 Da
• NCBI Official Full Name: lysophosphatidylcholine acyltransferase 2
• NCBI Official Synonym Full Names: lysophosphatidylcholine acyltransferase 2
• NCBI Official Symbol: Lpcat2
• NCBI Official Synonym Symbols: Aytl1; Aytl1a; lpafat1; A330042H22
• NCBI Protein Information: lysophosphatidylcholine acyltransferase 2
• UniProt Protein Name: Lysophosphatidylcholine acyltransferase 2
• Protein Family: Lysophosphatidylcholine acyltransferase
• UniProt Gene Name: Lpcat2
• UniProt Synonym Gene Names: Aytl1; Aytl1a; Lpcat2a; LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; 1-AGP acyltransferase 11; 1-AGPAT 11; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT
• UniProt Entry Name: PCAT2_MOUSE

Uniprot Description

AYTL1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet- activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso- PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.67; EC 2.3.1.51; Membrane protein, integral; EC 2.3.1.23; Acetyltransferase

Cellular Component: Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; integral to membrane; lipid particle

Molecular Function: transferase activity; 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; metal ion binding; transferase activity, transferring acyl groups; calcium ion binding; 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: platelet activating factor biosynthetic process; metabolic process; lipid metabolic process; phospholipid biosynthetic process