KCNE2 sirna

KCNE2 siRNA (Mouse)

Cat. Num. AAA8233839

• Gene Names: Kcne2; MiRP1; AW048273; 2200002I16Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNE2; KCNE2 siRNA (Mouse); Potassium voltage-gated channel subfamily E member 2; MinK-related peptide 1; Minimum potassium ion channel-related peptide 1; Potassium channel subunit beta MiRP1; KCNE2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KCNE2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 123

• Applicable Applications for KCNE2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KCNE2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNE2 sirna

siRNA to inhibit KCNE2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19882205
• NCBI GeneID: 246133
• NCBI Accession #: NP_598871.1
• NCBI GenBank Nucleotide #: NM_134110.3
• UniProt Accession #: Q9D808; Q8R1Z7
• Molecular Weight: 14,370 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily E member 2
• NCBI Official Synonym Full Names: potassium voltage-gated channel, Isk-related subfamily, gene 2
• NCBI Official Symbol: Kcne2
• NCBI Official Synonym Symbols: MiRP1; AW048273; 2200002I16Rik
• NCBI Protein Information: potassium voltage-gated channel subfamily E member 2
• UniProt Protein Name: Potassium voltage-gated channel subfamily E member 2
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcne2
• UniProt Entry Name: KCNE2_MOUSE

Uniprot Description

KCNE2: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with KCNQ1/KVLTQ1 and elicit a voltage-independent current. May associate with HCN1 and HCN2 and increase potassium current. Defects in KCNE2 are the cause of long QT syndrome type 6 (LQT6). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. KCNE2 mutants form channels that open slowly and close rapidly, thereby diminishing potassium currents. Defects in KCNE2 are the cause of familial atrial fibrillation type 4 (ATFB4). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the potassium channel KCNE family.

Protein type: Membrane protein, integral

Cellular Component: voltage-gated potassium channel complex; cell surface; membrane; lysosome; integral to membrane; plasma membrane

Molecular Function: voltage-gated potassium channel activity; protein binding; potassium channel regulator activity; protein homodimerization activity; potassium channel activity; delayed rectifier potassium channel activity; voltage-gated ion channel activity; inward rectifier potassium channel activity

Biological Process: potassium ion import; transport; ion transport; potassium ion transport