KCNA5 sirna

KCNA5 siRNA (Mouse)

Cat. Num. AAA8239207

• Gene Names: Kcna5; Kv1.5
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KCNA5; KCNA5 siRNA (Mouse); Potassium voltage-gated channel subfamily A member 5; Voltage-gated potassium channel subunit Kv1.5; KV1-5; KCNA5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KCNA5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 602

• Applicable Applications for KCNA5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KCNA5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KCNA5 sirna

siRNA to inhibit KCNA5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22122429
• NCBI GeneID: 16493
• NCBI Accession #: NP_666095.1
• NCBI GenBank Nucleotide #: NM_145983.2
• UniProt Accession #: Q61762; Q9Z1R6
• Molecular Weight: 56,871 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily A member 5
• NCBI Official Synonym Full Names: potassium voltage-gated channel, shaker-related subfamily, member 5
• NCBI Official Symbol: Kcna5
• NCBI Official Synonym Symbols: Kv1.5
• NCBI Protein Information: potassium voltage-gated channel subfamily A member 5
• UniProt Protein Name: Potassium voltage-gated channel subfamily A member 5
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcna5
• UniProt Synonym Gene Names: KV1-5
• UniProt Entry Name: KCNA5_MOUSE

Uniprot Description

Kv1.5: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. This channel displays rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets. Isoform 2 exhibits a voltage-dependent recovery from inactivation and an excessive cumulative inactivation. Defects in KCNA5 are the cause of familial atrial fibrillation type 7 (ATFB7). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.5/KCNA5 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; voltage-gated potassium channel complex; intracellular canaliculus; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; integral to plasma membrane; integral to membrane; plasma membrane; Z disc; cytosol; lipid raft

Molecular Function: voltage-gated potassium channel activity; protein binding; potassium channel activity; outward rectifier potassium channel activity; delayed rectifier potassium channel activity; ion channel activity; voltage-gated ion channel activity; potassium channel inhibitor activity; alpha-actinin binding; protein kinase binding; receptor binding

Biological Process: regulation of potassium ion transport; negative regulation of potassium ion transport; membrane hyperpolarization; protein oligomerization; regulation of membrane potential; transport; regulation of vasoconstriction; reduction of cytosolic calcium ion concentration; ion transport; potassium ion homeostasis; transmembrane transport; potassium ion transport; protein homooligomerization