Rabbit anti-Human RNF40 Polyclonal Antibody | anti-RNF40 antibody

RNF40 Polyclonal Antibody

Cat. Num. AAA3011577

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RNF40; Polyclonal Antibody; RNF40 Polyclonal Antibody; E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein; RBP95; RING finger protein 40; BRE1B; KIAA0661; anti-RNF40 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RNF40 polyclonal antibody detects endogenous levels of RNF40 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1001

• Applicable Applications for anti-RNF40 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:100; ChIP : 1:50-1:200
• Immunogen: Recombinant full length Human RNF40.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RNF40 polyclonal antibody)

Related Product Information for anti-RNF40 antibody

Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF40 (RING finger protein 40), also known as BRE1B, Staring or RBP95, is a 1, 001 amino acid nuclear protein that contains one RING-type zinc finger. Expressed ubiquitously with highest expression in heart, testis and pancreas, RNF40 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B and Syntaxin 1. In addition, RNF40 forms a ubiquitin ligase complex with UBCH6 (an E2 enzyme) and together, these proteins play a crucial role in regulation of the histone code. Four isoforms of RNF40 exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 313104295
• UniProt Accession #: O75150
• Molecular Weight: ~ 140kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase BRE1B
• UniProt Protein Name: E3 ubiquitin-protein ligase BRE1B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF40
• UniProt Synonym Gene Names: BRE1B; KIAA0661; BRE1-B; RBP95
• UniProt Entry Name: BRE1B_HUMAN

Uniprot Description

RNF40: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with RB1 and WAC. Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. Belongs to the BRE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 16p11.2-p11.1

Cellular Component: nucleoplasm; neuron projection; membrane; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; mRNA 3'-UTR binding; zinc ion binding; ubiquitin protein ligase binding; protein complex binding; syntaxin-1 binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: histone monoubiquitination; ubiquitin-dependent protein catabolic process; regulation of mitotic cell cycle; histone H2B ubiquitination