JMJD6 sirna

JMJD6 siRNA (Mouse)

Cat. Num. AAA828503

• Gene Names: Jmjd6; PSR; Ptdsr; PtdSerR; mKIAA0585; D11Ertd195e; 5730436I23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: JMJD6; JMJD6 siRNA (Mouse); KIAA0585; PTDSR; Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; Jumonji domain-containing protein 6; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptor; Protein PTDSR; JMJD6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: JMJD6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 403

• Applicable Applications for JMJD6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse JMJD6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for JMJD6 sirna

siRNA to inhibit JMJD6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 125988379
• NCBI GeneID: 107817
• NCBI Accession #: NP_203971.2
• NCBI GenBank Nucleotide #: NM_033398.2
• UniProt Accession #: Q9ERI5; Q80TX1; A2AA26; A8Y5I2
• Molecular Weight: 35,177 Da
• NCBI Official Full Name: bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• NCBI Official Synonym Full Names: jumonji domain containing 6
• NCBI Official Symbol: Jmjd6
• NCBI Official Synonym Symbols: PSR; Ptdsr; PtdSerR; mKIAA0585; D11Ertd195e; 5730436I23Rik
• NCBI Protein Information: bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• UniProt Protein Name: Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• Protein Family: Bifunctional arginine demethylase and lysyl-hydroxylase
• UniProt Gene Name: Jmjd6
• UniProt Synonym Gene Names: Kiaa0585; Ptdsr; Protein PTDSR
• UniProt Entry Name: JMJD6_MOUSE

NCBI Description

This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein functions in differentiation of multiple tissues during development, and in anti-inflammatory cytokine signaling. It was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that this protein does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

JMJD6: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. Belongs to the JMJD6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Apoptosis; Cell development/differentiation; Oxidoreductase; Nucleolus; Receptor, misc.

Cellular Component: nucleoplasm; nucleolus; plasma membrane; nucleus

Molecular Function: identical protein binding; histone demethylase activity (H4-R3 specific); RNA binding; metal ion binding; receptor activity; oxidoreductase activity; procollagen-proline dioxygenase activity; protein binding; 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity; single-stranded RNA binding; dioxygenase activity; sulfonate dioxygenase activity; iron ion binding; DNA-N1-methyladenine dioxygenase activity; histone demethylase activity (H3-R2 specific)

Biological Process: blood vessel development; erythrocyte development; transcription, DNA-dependent; heart development; RNA splicing; multicellular organismal development; T cell differentiation in the thymus; chromatin modification; recognition of apoptotic cell; peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine; regulation of nuclear mRNA splicing, via spliceosome; macrophage activation; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; retina development in camera-type eye; sprouting angiogenesis; mRNA processing; cell differentiation; kidney development; apoptotic cell clearance; lung development