ITGA6 sirna

ITGA6 siRNA (Mouse)

Cat. Num. AAA8231987

• Gene Names: Itga6; Cd49f; VLA-6; AI115430; 5033401O05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGA6; ITGA6 siRNA (Mouse); Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD antigen CD49f; ITGA6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ITGA6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1091

• Applicable Applications for ITGA6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ITGA6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGA6 sirna

siRNA to inhibit ITGA6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 485836844
• NCBI GeneID: 16403
• NCBI Accession #: NP_001264899.1
• NCBI GenBank Nucleotide #: NM_001277970.1
• UniProt Accession #: Q61739; A2AU04
• Molecular Weight: 119,606 Da
• NCBI Official Full Name: integrin alpha-6 isoform 2
• NCBI Official Synonym Full Names: integrin alpha 6
• NCBI Official Symbol: Itga6
• NCBI Official Synonym Symbols: Cd49f; VLA-6; AI115430; 5033401O05Rik
• NCBI Protein Information: integrin alpha-6
• UniProt Protein Name: Integrin alpha-6
• Protein Family: Integrin
• UniProt Gene Name: Itga6
• UniProt Entry Name: ITA6_MOUSE

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]

Uniprot Description

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: cell surface; focal adhesion; basolateral plasma membrane; integral to membrane; cell-cell adherens junction; hemidesmosome; membrane; plasma membrane; basal plasma membrane; basement membrane; integrin complex; external side of plasma membrane; filopodium

Molecular Function: integrin binding; metal ion binding; protein complex binding; laminin binding

Biological Process: integrin-mediated signaling pathway; skin development; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; odontogenesis of dentine-containing teeth; cell-cell adhesion; gut development; cellular response to extracellular stimulus; brown fat cell differentiation; positive regulation of cell-cell adhesion; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; leukocyte migration; positive regulation of phosphorylation