IL12A sirna

IL12A siRNA (Mouse)

Cat. Num. AAA8220643

• Gene Names: Il12a; p35; Ll12a; Il-12a; IL-12p35
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IL12A; IL12A siRNA (Mouse); Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; IL12A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: IL12A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 236

• Applicable Applications for IL12A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IL12A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IL12A sirna

siRNA to inhibit IL12A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226874945
• NCBI GeneID: 16159
• NCBI Accession #: NP_001152896.1
• NCBI GenBank Nucleotide #: NM_001159424.2
• UniProt Accession #: P43431
• Molecular Weight: 24,179 Da
• NCBI Official Full Name: interleukin-12 subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 12a
• NCBI Official Symbol: Il12a
• NCBI Official Synonym Symbols: p35; Ll12a; Il-12a; IL-12p35
• NCBI Protein Information: interleukin-12 subunit alpha
• UniProt Protein Name: Interleukin-12 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il12a
• UniProt Synonym Gene Names: IL-12A; CLMF p35
• UniProt Entry Name: IL12A_MOUSE

Uniprot Description

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; interleukin-12 complex; cytoplasm; extracellular region

Molecular Function: interleukin-27 binding; interleukin-12 beta subunit binding; growth factor activity; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding

Biological Process: positive regulation of NK T cell activation; cell migration; positive regulation of cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; negative regulation of smooth muscle cell proliferation; positive regulation of T cell mediated cytotoxicity; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; defense response to protozoan; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell activation; response to UV-B; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of T cell differentiation; positive regulation of mononuclear cell proliferation; positive regulation of lymphocyte proliferation; immune response; positive regulation of T cell proliferation; cell cycle arrest