HSPG2 sirna

HSPG2 siRNA (Mouse)

Cat. Num. AAA8217647

• Gene Names: Hspg2; Pcn; Plc; per; AI852380
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSPG2; HSPG2 siRNA (Mouse); Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; HSPG2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HSPG2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3,707

• Applicable Applications for HSPG2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HSPG2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSPG2 sirna

siRNA to inhibit HSPG2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 183979966
• NCBI GeneID: 15530
• NCBI Accession #: NP_032331.2
• NCBI GenBank Nucleotide #: NM_008305.3
• UniProt Accession #: Q05793
• Molecular Weight: 398,294 Da
• NCBI Official Full Name: basement membrane-specific heparan sulfate proteoglycan core protein
• NCBI Official Synonym Full Names: perlecan (heparan sulfate proteoglycan 2)
• NCBI Official Symbol: Hspg2
• NCBI Official Synonym Symbols: Pcn; Plc; per; AI852380
• NCBI Protein Information: basement membrane-specific heparan sulfate proteoglycan core protein
• UniProt Protein Name: Basement membrane-specific heparan sulfate proteoglycan core protein
• Protein Family: Small heat shock protein
• UniProt Gene Name: Hspg2
• UniProt Synonym Gene Names: HSPG
• UniProt Entry Name: PGBM_MOUSE

Uniprot Description

HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; focal adhesion; extracellular region; basement membrane; basal lamina

Molecular Function: protein C-terminus binding; protein binding; protease binding; metal ion binding

Biological Process: cardiac muscle development; extracellular matrix organization and biogenesis; protein localization; chondrocyte differentiation; brain development; angiogenesis; endochondral ossification; embryonic skeletal morphogenesis