HSPA8 sirna

HSPA8 siRNA (Mouse)

Cat. Num. AAA8207857

• Gene Names: Hspa8; Hsc70; Hsc71; Hsc73; Hsp73; Hspa10; 2410008N15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSPA8; HSPA8 siRNA (Mouse); HSC70; HSC73; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; HSPA8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HSPA8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 646

• Applicable Applications for HSPA8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HSPA8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSPA8 sirna

siRNA to inhibit HSPA8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31981690
• NCBI GeneID: 15481
• NCBI Accession #: NP_112442.2
• NCBI GenBank Nucleotide #: NM_031165.4
• UniProt Accession #: P63017; P08109; P12225; Q3U6R0; Q3U764; Q3U7D7; Q3U7E2; Q3U9B4; Q3U9G0; Q3UGM0; Q5FWJ6; Q62373
• Molecular Weight: 70,871 Da
• NCBI Official Full Name: heat shock cognate 71 kDa protein
• NCBI Official Synonym Full Names: heat shock protein 8
• NCBI Official Symbol: Hspa8
• NCBI Official Synonym Symbols: Hsc70; Hsc71; Hsc73; Hsp73; Hspa10; 2410008N15Rik
• NCBI Protein Information: heat shock cognate 71 kDa protein
• UniProt Protein Name: Heat shock cognate 71 kDa protein
• UniProt Gene Name: Hspa8
• UniProt Synonym Gene Names: Hsc70; Hsc73
• UniProt Entry Name: HSP7C_MOUSE

Uniprot Description

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone; RNA-binding; Heat shock protein; Nucleolus

Cellular Component: extracellular space; neuron projection; protein complex; focal adhesion; terminal button; ribonucleoprotein complex; cytosol; spliceosome; synaptic vesicle; cell soma; membrane; cytoplasm; plasma membrane; intracellular; nucleus; myelin sheath; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; heat shock protein binding; ATPase activity; unfolded protein binding; nucleotide binding; ATPase activity, coupled; ADP binding; ATP binding; receptor binding

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; transcription, DNA-dependent; protein folding; chaperone cofactor-dependent protein folding; regulation of cell cycle; RNA splicing; protein refolding; mRNA processing; negative regulation of transcription, DNA-dependent