HSP90AA1 sirna

HSP90AA1 siRNA (Mouse)

Cat. Num. AAA8219011

• Gene Names: Hsp90aa1; hsp4; 86kDa; 89kDa; Hsp89; Hsp90; Hspca; Hsp86-1; AL024080; AL024147
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSP90AA1; HSP90AA1 siRNA (Mouse); HSP86; HSP86-1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; Tumor-specific transplantation 86 kDa antigen; TSTA; HSP90AA1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HSP90AA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 733

• Applicable Applications for HSP90AA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HSP90AA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSP90AA1 sirna

siRNA to inhibit HSP90AA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754254
• NCBI GeneID: 15519
• NCBI Accession #: NP_034610.1
• NCBI GenBank Nucleotide #: NM_010480.5
• UniProt Accession #: P07901
• Molecular Weight: 84,788 Da
• NCBI Official Full Name: heat shock protein HSP 90-alpha
• NCBI Official Synonym Full Names: heat shock protein 90, alpha (cytosolic), class A member 1
• NCBI Official Symbol: Hsp90aa1
• NCBI Official Synonym Symbols: hsp4; 86kDa; 89kDa; Hsp89; Hsp90; Hspca; Hsp86-1; AL024080; AL024147
• NCBI Protein Information: heat shock protein HSP 90-alpha
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• Protein Family: Heat shock protein
• UniProt Gene Name: Hsp90aa1
• UniProt Synonym Gene Names: Hsp86; Hsp86-1; Hspca; HSP 86; HSP86; TSTA
• UniProt Entry Name: HS90A_MOUSE

Uniprot Description

HSP90A: a molecular chaperone of the heat shock protein 90 family. Has ATPase activity. Known to interact with a wide variety of proteins including steroid hormone receptors, neuropeptide Y, FKBP51/54, and FKBP52. G protein-coupled receptor kinases are stabilized by interacting with HSP 90. Hsp70 and Hsp90 promote tau solubility and tau binding to microtubules, reducing insoluble tau phosphorylation of tau.

Protein type: Chaperone; Heat shock protein

Cellular Component: neuron projection; protein complex; cell surface; mitochondrion; basolateral plasma membrane; brush border membrane; cytosol; nucleoplasm; extracellular matrix; membrane; cell soma; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; intracellular; nucleus; myelin sheath

Molecular Function: identical protein binding; protein homodimerization activity; GTP binding; ATPase activity; unfolded protein binding; nitric-oxide synthase regulator activity; nucleotide binding; dATP binding; sulfonylurea receptor binding; protein phosphatase binding; protein kinase binding; mRNA binding; protein binding; TPR domain binding; Rho GDP-dissociation inhibitor binding; ATP binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of protein import into nucleus, translocation; positive regulation of cell size; protein folding; neuron migration; protein import into mitochondrial outer membrane; response to unfolded protein; positive regulation of cytotoxic T cell differentiation; regulation of catalytic activity; response to stress; protein refolding; chaperone-mediated protein complex assembly; nitric oxide biosynthetic process