HRH2 sirna

HRH2 siRNA (Mouse)

Cat. Num. AAA8228077

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HRH2; HRH2 siRNA (Mouse); Histamine H2 receptor; H2R; HH2R; Gastric receptor I; HRH2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HRH2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 397

• Applicable Applications for HRH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HRH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HRH2 sirna

siRNA to inhibit HRH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58331134
• NCBI GeneID: 15466
• NCBI Accession #: NP_001010973.1
• NCBI GenBank Nucleotide #: NM_001010973.2
• UniProt Accession #: P97292; Q9D282
• Molecular Weight: 44,794 Da
• NCBI Official Full Name: histamine H2 receptor isoform 1
• NCBI Official Synonym Full Names: histamine receptor H2
• NCBI Official Symbol: Hrh2
• NCBI Protein Information: histamine H2 receptor
• UniProt Protein Name: Histamine H2 receptor
• Protein Family: Histamine H2 receptor
• UniProt Gene Name: Hrh2
• UniProt Synonym Gene Names: H2R; HH2R
• UniProt Entry Name: HRH2_MOUSE

Uniprot Description

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; histamine receptor activity

Biological Process: gastric acid secretion; gland development; G-protein coupled receptor protein signaling pathway; gastrin-induced gastric acid secretion; gut development; histamine-induced gastric acid secretion; positive regulation of vasoconstriction; regulation of synaptic plasticity; visual learning; signal transduction; memory