Rabbit anti-Human, Rat MMP12 Polyclonal Antibody | anti-MMP12 antibody

MMP12 (Macrophage Metalloelastase, MME, Macrophage Elastase, ME, hME, Matrix Metalloproteinase-12, MMP-12, HME, MGC138506) (Biotin)

Cat. Num. AAA6385612

• Gene Names: MMP12; ME; HME; MME; MMP-12
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MMP12; Polyclonal Antibody; MMP12 (Macrophage Metalloelastase; MME; Macrophage Elastase; ME; hME; Matrix Metalloproteinase-12; MMP-12; HME; MGC138506) (Biotin); anti-MMP12 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP12. Species Crossreactivity: rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MMP12 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human MMP12, aa1-470 (NP_002417.2).
• Immunogen Sequence: MKFLLILLLQATASGALPLNSSTSLEKNNVLFGERYLEKFYGLEINKLPVTKMKYSGNLMKEKIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDVHHFREMPGGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFFFKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFEYDFLLQRITKTLKSNSWFGC
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MMP12 rabbit polyclonal antibody. Western Blot analysis of MMP12 expression in HeLa.)

Western Blot (WB)

(MMP12 rabbit polyclonal antibody. Western Blot analysis of MMP12 expression in PC-12.)

Western Blot (WB)

(Western Blot analysis of MMP12 expression in transfected 293T cell line by MMP12 polyclonal antibody. Lane 1: MMP12 transfected lysate (54kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-MMP12 antibody

Human macrophage elastase is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. MMP-12 expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase. Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms.

Product Categories/Family for anti-MMP12 antibody

Antibodies; Matrix Metalloproteinases

NCBI and Uniprot Product Information

• NCBI GI #: 73858572
• NCBI GeneID: 4321
• NCBI Accession #: NP_002417.2
• NCBI GenBank Nucleotide #: NM_002426
• UniProt Accession #: P39900; Q2M1L9; B2R9X8; B7ZLF6
• Molecular Weight: 54,002 Da
• NCBI Official Full Name: macrophage metalloelastase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 12 (macrophage elastase)
• NCBI Official Symbol: MMP12
• NCBI Official Synonym Symbols: ME; HME; MME; MMP-12
• NCBI Protein Information: macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase)
• UniProt Protein Name: Macrophage metalloelastase
• Protein Family: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12
• UniProt Entry Name: MMP12_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.65; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells