HPSE sirna

HPSE siRNA (Mouse)

Cat. Num. AAA8207803

• Gene Names: Hpse; Hpa; HSE1; Hpr1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HPSE; HPSE siRNA (Mouse); HPA; Heparanase; Endo-glucoronidase; HPSE sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HPSE siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 535

• Applicable Applications for HPSE sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HPSE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HPSE sirna

siRNA to inhibit HPSE expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22779887
• NCBI GeneID: 15442
• NCBI Accession #: NP_690016.1
• NCBI GenBank Nucleotide #: NM_152803.4
• UniProt Accession #: Q6YGZ1; Q8K3K3; B2RS99
• Molecular Weight: 60,066 Da
• NCBI Official Full Name: heparanase
• NCBI Official Synonym Full Names: heparanase
• NCBI Official Symbol: Hpse
• NCBI Official Synonym Symbols: Hpa; HSE1; Hpr1
• NCBI Protein Information: heparanase
• UniProt Protein Name: Heparanase
• Protein Family: Heparanase
• UniProt Gene Name: Hpse
• UniProt Synonym Gene Names: Hpa
• UniProt Entry Name: HPSE_MOUSE

Uniprot Description

HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.

Protein type: EC 3.2.1.166; Hydrolase; Secreted, signal peptide; Secreted; Extracellular matrix; Glycan Metabolism - glycosaminoglycan degradation

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; membrane; intracellular membrane-bound organelle; lysosome; extracellular region; nucleus; lipid raft

Molecular Function: syndecan binding; protein dimerization activity; hydrolase activity; heparanase activity; hydrolase activity, acting on glycosyl bonds

Biological Process: positive regulation of hair follicle development; positive regulation of protein kinase B signaling cascade; positive regulation of blood coagulation; heparan sulfate proteoglycan catabolic process; wound healing; cell-matrix adhesion; carbohydrate metabolic process; positive regulation of osteoblast proliferation; regulation of hair follicle development; cell adhesion; angiogenesis involved in wound healing