HIPK3 sirna

HIPK3 siRNA (Mouse)

Cat. Num. AAA829361

• Gene Names: Hipk3; PKY; DYRK6; FIST3; mir-1902
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HIPK3; HIPK3 siRNA (Mouse); FIST3; Homeodomain-interacting protein kinase 3; Androgen receptor-interacting nuclear protein kinase; ANPK; Fas-interacting serine/threonine-protein kinase; FIST; Nuclear body-associated kinase 3; Nbak3; HIPK3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HIPK3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1192

• Applicable Applications for HIPK3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HIPK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HIPK3 sirna

siRNA to inhibit HIPK3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224922824
• NCBI GeneID: 15259
• NCBI Accession #: NP_034564.2
• NCBI GenBank Nucleotide #: NM_010434.2
• UniProt Accession #: Q9ERH7; O88906; Q9QZR2; A2AQH2
• Molecular Weight: 130,081 Da
• NCBI Official Full Name: homeodomain-interacting protein kinase 3 isoform 1
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 3
• NCBI Official Symbol: Hipk3
• NCBI Official Synonym Symbols: PKY; DYRK6; FIST3; mir-1902
• NCBI Protein Information: homeodomain-interacting protein kinase 3
• UniProt Protein Name: Homeodomain-interacting protein kinase 3
• Protein Family: Homeodomain-interacting protein kinase
• UniProt Gene Name: Hipk3
• UniProt Synonym Gene Names: Fist3; ANPK; FIST; Nbak3
• UniProt Entry Name: HIPK3_MOUSE

Uniprot Description

HIPK3: homeodomain interacting protein kinase 3 is a CMGC kinase of the DYRK family. Phosphorylates FADD and inhibits fas-mediated Jun NH(2)-terminal kinase activation.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Nuclear receptor co-regulator; Protein kinase, CMGC; Kinase, protein; CMGC group; DYRK family; HIPK subfamily

Cellular Component: PML body; cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; DNA binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transcription corepressor activity; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK activity; peptidyl-serine phosphorylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; mRNA transcription; peptidyl-threonine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; phosphorylation; protein amino acid phosphorylation; positive regulation of DNA binding; negative regulation of apoptosis