Western Blot (WB)
(Western blot analysis of NIFK in 30 ugs of NIH3T3 cell lysate using IMG-560 at 1:1000.)
Rabbit anti-Mouse NIFK, aa75-90 Polyclonal Antibody | anti-nifK antibody
NIFK, Mouse, aa75-90 (Ki-67ip)
Reactivity
Mouse
Applications
Western Blot
Purity
PurifiedPurified
Synonyms
NIFK; aa75-90; Polyclonal Antibody; Mouse; aa75-90 (Ki-67ip); Anti -NIFK; anti-nifK antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse NIFK, Mouse, aa75-90 (Ki67ip)
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Applicable Applications for anti-nifK antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500-1:1000
observed size: 34 kD
positive control: NIH3T3 whole cell lysate
Dilution: Western Blot: 1:500-1:1000
observed size: 34 kD
positive control: NIH3T3 whole cell lysate
Immunogen
Synthetic peptide corresponding to amino acids 75-90 of mouse NIFK.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of NIFK in 30 ugs of NIH3T3 cell lysate using IMG-560 at 1:1000.)
Western Blot (WB)
(Western blot analysis of NIFK in 30 ugs of NIH3T3 cell lysate using IMG-560 at 1:1000.)
Related Product Information for anti-nifK antibody
NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.
Product Categories/Family for anti-nifK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nitrogenase FeMo beta subunit protein; NifK
NCBI Official Symbol
nifK
NCBI Protein Information
nitrogenase FeMo beta subunit protein; NifK
Protein Family
Similar Products
Product Notes
The nifK (Catalog #AAA617335) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NIFK, Mouse, aa75-90 (Ki-67ip) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NIFK, aa75-90 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:500-1:1000 observed size: 34 kD positive control: NIH3T3 whole cell lysate. Researchers should empirically determine the suitability of the nifK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIFK, aa75-90, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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