HELLS sirna

HELLS siRNA (Mouse)

Cat. Num. AAA829563

• Gene Names: Hells; LSH; Lysh; PASG; YFK8; AI323785; E130115I21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HELLS; HELLS siRNA (Mouse); LSH; PASG; Lymphocyte-specific helicase; Proliferation-associated SNF2-like protein; HELLS sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HELLS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 821

• Applicable Applications for HELLS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HELLS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HELLS sirna

siRNA to inhibit HELLS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12232371
• NCBI GeneID: 15201
• NCBI Accession #: NP_032260.2
• NCBI GenBank Nucleotide #: NM_008234.3
• UniProt Accession #: Q60848; Q497T3; Q8VDZ1; Q9CYV7
• Molecular Weight: 93,786 Da
• NCBI Official Full Name: lymphocyte-specific helicase
• NCBI Official Synonym Full Names: helicase, lymphoid specific
• NCBI Official Symbol: Hells
• NCBI Official Synonym Symbols: LSH; Lysh; PASG; YFK8; AI323785; E130115I21Rik
• NCBI Protein Information: lymphocyte-specific helicase
• UniProt Protein Name: Lymphocyte-specific helicase
• Protein Family: Lymphoid-specific helicase
• UniProt Gene Name: Hells
• UniProt Entry Name: HELLS_MOUSE

Uniprot Description

HELLS: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. By concanavalin-A in peripheral blood leukocytes. Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. Belongs to the SNF2/RAD54 helicase family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.6.1.-; EC 3.6.4.-; Helicase

Cellular Component: centric heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; hydrolase activity; nucleotide binding; chromatin binding; helicase activity; ATP binding

Biological Process: mitosis; maintenance of DNA methylation; transcription, DNA-dependent; multicellular organismal development; chromatin silencing; cell cycle; lymphocyte differentiation; regulation of transcription, DNA-dependent; methylation-dependent chromatin silencing; cell division; centric heterochromatin formation; DNA methylation; urogenital system development; negative regulation of apoptosis