H2AFY sirna

H2AFY siRNA (Mouse)

Cat. Num. AAA8234480

• Gene Names: H2afy; mH2a1; H2AF12M; macroH2A1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: H2AFY; H2AFY siRNA (Mouse); Core histone macro-H2A.1; Histone macroH2A1; mH2A1; H2A.y; H2A/y; H2AFY sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: H2AFY siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 371

• Applicable Applications for H2AFY sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse H2AFY gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for H2AFY sirna

siRNA to inhibit H2AFY expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 283945572
• NCBI GeneID: 26914
• NCBI Accession #: NP_001152985.1
• NCBI GenBank Nucleotide #: NM_001159513.1
• UniProt Accession #: Q9QZQ8; Q91VZ2; Q9QZQ7
• Molecular Weight: 39,290 Da
• NCBI Official Full Name: core histone macro-H2A.1 isoform 2
• NCBI Official Synonym Full Names: H2A histone family, member Y
• NCBI Official Symbol: H2afy
• NCBI Official Synonym Symbols: mH2a1; H2AF12M; macroH2A1
• NCBI Protein Information: core histone macro-H2A.1
• UniProt Protein Name: Core histone macro-H2A.1
• Protein Family: Core histone macro
• UniProt Gene Name: H2afy
• UniProt Synonym Gene Names: Histone macroH2A1; mH2A1
• UniProt Entry Name: H2AY_MOUSE

Uniprot Description

H2AFY: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: nucleoplasm; centric heterochromatin; centrosome; ESC/E(Z) complex; nuclear chromatin; nucleolus; Barr body; condensed chromosome; nucleosome; chromosome; chromatin; nucleus

Molecular Function: protein serine/threonine kinase inhibitor activity; rDNA binding; DNA binding; double-stranded methylated DNA binding; chromatin DNA binding; protein heterodimerization activity; chromatin binding; protein kinase binding

Biological Process: nucleosome assembly; negative regulation of gene expression, epigenetic; dosage compensation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of histone phosphorylation