GRIN2B sirna

GRIN2B siRNA (Mouse)

Cat. Num. AAA8215037

• Gene Names: Grin2b; NR2B; GluN2B; Nmdar2b; AW490526
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GRIN2B; GRIN2B siRNA (Mouse); Glutamate receptor ionotropic. NMDA 2B; GluN2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; GRIN2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GRIN2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1482

• Applicable Applications for GRIN2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GRIN2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GRIN2B sirna

siRNA to inhibit GRIN2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 117168299
• NCBI GeneID: 14812
• NCBI Accession #: NP_032197.3
• NCBI GenBank Nucleotide #: NM_008171.3
• UniProt Accession #: Q01097; Q9DCB2
• Molecular Weight: 165,959 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2B
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
• NCBI Official Symbol: Grin2b
• NCBI Official Synonym Symbols: NR2B; GluN2B; Nmdar2b; AW490526
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2B
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2B
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2b
• UniProt Synonym Gene Names: GluN2B; NMDAR2B; NR2B
• UniProt Entry Name: NMDE2_MOUSE

Uniprot Description

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: neuron projection; cell surface; postsynaptic density; integral to membrane; dendritic spine; terminal button; Z disc; N-methyl-D-aspartate selective glutamate receptor complex; presynaptic membrane; postsynaptic membrane; synaptic vesicle; membrane; plasma membrane; synapse; cell junction

Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; metal ion binding; calcium channel activity; ion channel activity; beta-catenin binding; receptor activity; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; cell adhesion molecule binding; cation channel activity; D2 dopamine receptor binding; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; startle response; behavioral fear response; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; receptor clustering; positive regulation of synaptic transmission; synaptic transmission; behavioral response to pain; learning and/or memory; transport; calcium ion transport; cation transport; associative learning; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; learning; memory; detection of mechanical stimulus involved in sensory perception of pain; embryonic development; regulation of postsynaptic membrane potential; response to ethanol; sensory organ development; suckling behavior; ionotropic glutamate receptor signaling pathway; regulation of MAPKKK cascade; ion transport; regulation of excitatory postsynaptic membrane potential; regulation of neuronal synaptic plasticity