Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1474) antibody.)
NMDAR2B Antibody | anti-NMDAR2B antibody
NMDAR2B (Phospho-Tyr1474) Antibody
Gene Names
Grin2b; NR2B; GluN2B; Nmdar2b; AW490526
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
NMDAR2B; NMDAR2B (Phospho-Tyr1474) Antibody; GRIN2B; Glutamate [NMDA] receptor subunit epsilon 2 precursor; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDE2; NME2; NR2B; NR3; hNR3; anti-NMDAR2B antibody
Reactivity
Human, Mouse, Rat
Specificity
NMDAR2B (Phospho-Tyr1474) antibody detects endogenous levels of NMDAR2B only when phosphorylated at tyrosine 1474.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1482
Applicable Applications for anti-NMDAR2B antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000
IHC: 1:50~1:100
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NMDAR2B around the phosphorylation site of tyrosine 1474 (H-V-YP-E-K).
All Sites
Human: Tyr1474
Mouse: Tyr1472
Rat: Tyr1472
Mouse: Tyr1472
Rat: Tyr1472
Preparation and Storage
Store at-20 degree C for 1 year.
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1474) antibody.)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using NMDAR2B (Phospho-Tyr1474) antibody.)
Product Categories/Family for anti-NMDAR2B antibody
References
Carola G. Schipke, FASEB J, Mar 2001; 10.1096/fj.00-0439fje. Myoung Sook Kim, Cancer Res., Apr 2006; 66: 3409-3418. MA Varney, J. Pharmacol. Exp. Ther., Oct 1996; 279: 367. Nobuhiko Kojima, Learn. Mem., Nov 1998; 5: 429-445.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
165,959 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, NMDA2B (epsilon 2)
NCBI Official Symbol
Grin2b
NCBI Official Synonym Symbols
NR2B; GluN2B; Nmdar2b; AW490526
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Gene Name
Grin2b
UniProt Synonym Gene Names
NMDAR2B; NR2B
Uniprot Description
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+ (PubMed:1377365, PubMed:26912815). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:1377365). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (PubMed:20141836). Contributes to neural pattern formation in the developing brain (PubMed:8789948). Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (PubMed:8789948).
Research Articles on NMDAR2B
Similar Products
Product Notes
The NMDAR2B grin2b (Catalog #AAA859299) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NMDAR2B (Phospho-Tyr1474) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NMDAR2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the NMDAR2B grin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMDAR2B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
