GRIN2A sirna

GRIN2A siRNA (Mouse)

Cat. Num. AAA8213467

• Gene Names: Grin2a; NR2A; GluN2A; NMDAR2A
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GRIN2A; GRIN2A siRNA (Mouse); Glutamate receptor ionotropic. NMDA 2A; GluN2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; GRIN2A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GRIN2A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1464

• Applicable Applications for GRIN2A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GRIN2A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GRIN2A sirna

siRNA to inhibit GRIN2A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41680705
• NCBI GeneID: 14811
• NCBI Accession #: NP_032196.2
• NCBI GenBank Nucleotide #: NM_008170.2
• UniProt Accession #: P35436
• Molecular Weight: 165,421 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, NMDA2A (epsilon 1)
• NCBI Official Symbol: Grin2a
• NCBI Official Synonym Symbols: NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2a
• UniProt Synonym Gene Names: GluN2A; NMDAR2A; NR2A
• UniProt Entry Name: NMDE1_MOUSE

Uniprot Description

NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral

Cellular Component: neuron projection; cell surface; endoplasmic reticulum; postsynaptic density; dendritic spine; integral to membrane; terminal button; N-methyl-D-aspartate selective glutamate receptor complex; presynaptic membrane; postsynaptic membrane; synaptic vesicle; membrane; plasma membrane; synapse; cell junction

Molecular Function: protein dimerization activity; voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; zinc ion binding; metal ion binding; calcium channel activity; ion channel activity; receptor activity; protein kinase binding; ATPase binding; protein binding; glutamate binding; extracellular-glutamate-gated ion channel activity; protein heterodimerization activity; ionotropic glutamate receptor activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; cell adhesion molecule binding; cation channel activity; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; startle response; positive regulation of apoptosis; rhythmic process; regulation of synaptic plasticity; locomotory behavior; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; calcium ion transport; response to wounding; visual learning; locomotion; serotonin metabolic process; protein tetramerization; cation transport; regulation of synaptic transmission; response to drug; synaptic transmission, glutamatergic; regulation of action potential; response to amphetamine; learning; sleep; memory; detection of mechanical stimulus involved in sensory perception of pain; regulation of membrane potential; response to ethanol; regulation of postsynaptic membrane potential; neurogenesis; ionotropic glutamate receptor signaling pathway; directional locomotion; ion transport; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process