Chicken Annexin A7 ELISA Kit | ANXA7 elisa kit

Chicken Annexin A7 ELISA Kit

Cat. Num. AAA747186

• Gene Names: ANXA7; SNX; ANX7; SYNEXIN
• Reactivity: Chicken
• Synonyms: Annexin A7; Chicken Annexin A7 ELISA Kit; ANXA7 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ANXA7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ANXA7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA7 elisa kit

Principle of the assay: ANXA7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA7 antibody and an ANXA7-HRP conjugate. The assay sample and buffer are incubated together with ANXA7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA7 concentration since ANXA7 from samples and ANXA7-HRP conjugate compete for the anti-ANXA7 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA7 from the sample, fewer sites are left to bind ANXA7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ANXA7 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4809279
• NCBI GeneID: 310
• NCBI Accession #: NP_004025.1
• NCBI GenBank Nucleotide #: NM_004034.2
• Molecular Weight: 50,316 Da
• NCBI Official Full Name: annexin A7 isoform 2
• NCBI Official Synonym Full Names: annexin A7
• NCBI Official Symbol: ANXA7
• NCBI Official Synonym Symbols: SNX; ANX7; SYNEXIN
• NCBI Protein Information: annexin A7; annexin-7; annexin VII
• UniProt Protein Name: Annexin A7
• Protein Family: Annexin
• UniProt Gene Name: ANXA7
• UniProt Synonym Gene Names: ANX7; SNX; OK/SW-cl.95
• UniProt Entry Name: ANXA7_HUMAN

NCBI Description

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA7: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Interacts with PDCD6. Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Belongs to the annexin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; nuclear envelope; cytosol; nucleus

Molecular Function: integrin binding; protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: cellular calcium ion homeostasis; cell proliferation; regulation of cell shape; cellular water homeostasis; epithelial cell differentiation; hemostasis; autophagy; social behavior; response to calcium ion; response to organic cyclic substance; response to salt stress