GNAS sirna

GNAS siRNA (Mouse)

Cat. Num. AAA8215418

• Gene Names: Gnas; P1; P2; P3; GSP; Gsa; POH; GPSA; Nesp; SCG6; Gnas1; Nespl; PHP1A; PHP1B; Gnasxl; Nesp55; Oedsml; Galphas; Oed-Sml; Gs-alpha; 5530400H20Rik; A930027G11Rik; C130027O20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GNAS; GNAS siRNA (Mouse); GNAS1; Neuroendocrine secretory protein 55; NESP55; GNAS sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GNAS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 253

• Applicable Applications for GNAS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GNAS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GNAS sirna

siRNA to inhibit GNAS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 117959892
• NCBI GeneID: 14683
• NCBI Accession #: NP_062664.2
• NCBI GenBank Nucleotide #: NM_019690.3
• UniProt Accession #: Q9Z0F1; Q9QXW5; Q9Z0H2; P63094; Q6R0H6; Q6R0H7
• Molecular Weight: 121,505 Da
• NCBI Official Full Name: protein SCG6 (secretogranin VI) isoform SCG6
• NCBI Official Synonym Full Names: GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus
• NCBI Official Symbol: Gnas
• NCBI Official Synonym Symbols: P1; P2; P3; GSP; Gsa; POH; GPSA; Nesp; SCG6; Gnas1; Nespl; PHP1A; PHP1B; Gnasxl; Nesp55; Oedsml; Galphas; Oed-Sml; Gs-alpha; 5530400H20Rik; A930027G11Rik; C130027O20Rik
• NCBI Protein Information: protein ALEX; protein GNAS; protein SCG6 (secretogranin VI)
• UniProt Protein Name: Neuroendocrine secretory protein 55
• Protein Family: Protein
• UniProt Gene Name: Gnas
• UniProt Synonym Gene Names: NESP55
• UniProt Entry Name: GNAS3_MOUSE

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, which is commonly found in imprinted genes and correlates with transcript expression. This gene has an antisense transcript. One of the transcripts produced from this locus, and the antisense transcript, are both paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Additional transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2015]

Uniprot Description

G-alpha(s) iso8: a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: G protein; Membrane protein, peripheral; Oncoprotein; Vesicle; G protein, heterotrimeric; G protein, heterotrimeric alpha G(s)

Cellular Component: dendrite; extracellular region; cytosol; lipid raft; ruffle; cell projection; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; trans-Golgi network membrane; intrinsic to membrane; cytoplasmic vesicle; nucleus; vesicle; endosome; sarcolemma

Molecular Function: GTPase activity; corticotropin-releasing hormone receptor 1 binding; protein domain specific binding; GTP binding; guanyl nucleotide binding; metal ion binding; nucleotide binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; ionotropic glutamate receptor binding; insulin-like growth factor receptor binding; signal transducer activity; protein binding; mu-type opioid receptor binding; G-protein beta/gamma-subunit binding; beta-2 adrenergic receptor binding; D1 dopamine receptor binding; alpha-tubulin binding

Biological Process: skin development; developmental growth; positive regulation of osteoclast differentiation; signal transduction; G-protein signaling, adenylate cyclase activating pathway; embryonic hindlimb morphogenesis; post-embryonic development; calcium ion homeostasis; positive regulation of cAMP biosynthetic process; tissue homeostasis; DNA methylation; negative regulation of blood pressure; skeletal development; post-embryonic body morphogenesis; dopamine receptor, adenylate cyclase activating pathway; endochondral ossification; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; embryonic cranial skeleton morphogenesis; regulation of transcription from RNA polymerase II promoter; G-protein coupled receptor protein signaling pathway; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; positive regulation of protein amino acid phosphorylation; cognition