GLIS3 sirna

GLIS3 siRNA (Mouse)

Cat. Num. AAA8222370

• Gene Names: Glis3; 4833409N03Rik; E330013K21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GLIS3; GLIS3 siRNA (Mouse); Zinc finger protein GLIS3; GLI-similar 3; GLIS3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GLIS3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 780

• Applicable Applications for GLIS3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GLIS3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GLIS3 sirna

siRNA to inhibit GLIS3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 569007036
• NCBI GeneID: 226075
• NCBI Accession #: XP_006527048.1
• NCBI GenBank Nucleotide #: XM_006526985.2
• UniProt Accession #: Q6XP49; Q8BI60
• Molecular Weight: 72,374 Da
• NCBI Official Full Name: zinc finger protein GLIS3 isoform X2
• NCBI Official Synonym Full Names: GLIS family zinc finger 3
• NCBI Official Symbol: Glis3
• NCBI Official Synonym Symbols: 4833409N03Rik; E330013K21Rik
• NCBI Protein Information: zinc finger protein GLIS3
• UniProt Protein Name: Zinc finger protein GLIS3
• Protein Family: Zinc finger protein
• UniProt Gene Name: Glis3
• UniProt Entry Name: GLIS3_MOUSE

NCBI Description

This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein which contains multiple C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the transcriptional regulation of insulin. It is thought to enhance GLI-RE-dependent transcription by binding to the GLI-RE consensus sequence (GACCACCCAC). Mutations in a similar gene in human have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

Uniprot Description

GLIS3: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Defects in GLIS3 are a cause of diabetes mellitus neonatal with congenital hypothyroidism (NDH). NDH is a neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor

Cellular Component: nucleoplasm; Golgi apparatus; nucleus

Molecular Function: DNA binding; nucleic acid binding; metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter