FZR1 sirna

FZR1 siRNA (Mouse)

Cat. Num. AAA8235437

• Gene Names: Fzr1; FZR; Fyr; Cdh1; FZR2; HCDH; HCDH1; AW108046
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FZR1; FZR1 siRNA (Mouse); FYR; FZR; Fizzy-related protein homolog; Fzr; Cdh1/Hct1 homolog; FZR1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FZR1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 493

• Applicable Applications for FZR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FZR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FZR1 sirna

siRNA to inhibit FZR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9789959
• NCBI GeneID: 56371
• NCBI Accession #: NP_062731.1
• NCBI GenBank Nucleotide #: NM_019757.1
• UniProt Accession #: Q9R1K5
• Molecular Weight: 54,689 Da
• NCBI Official Full Name: fizzy-related protein homolog
• NCBI Official Synonym Full Names: fizzy/cell division cycle 20 related 1 (Drosophila)
• NCBI Official Symbol: Fzr1
• NCBI Official Synonym Symbols: FZR; Fyr; Cdh1; FZR2; HCDH; HCDH1; AW108046
• NCBI Protein Information: fizzy-related protein homolog
• UniProt Protein Name: Fizzy-related protein homolog
• Protein Family: Fizzy-related protein
• UniProt Gene Name: Fzr1
• UniProt Synonym Gene Names: Fyr; Fzr; Fzr
• UniProt Entry Name: FZR_MOUSE

Uniprot Description

FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Cellular Component: nucleoplasm; nuclear membrane; anaphase-promoting complex

Molecular Function: protein binding

Biological Process: mitosis; anaphase-promoting complex activation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell proliferation; positive regulation of protein catabolic process; regulation of meiosis; DNA repair; cell cycle; G2/M transition DNA damage checkpoint; response to DNA damage stimulus