Mouse NME4 Monoclonal Antibody | anti-NME4 antibody

NME4 antibody

Cat. Num. AAA834513

• Gene Names: NME4; NDPK-D; NM23H4; nm23-H4
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: NME4 antibody was purified by affinity chromatography.
• Synonyms: NME4; Monoclonal Antibody; NME4 antibody; Monoclonal NME4; Anti-NME4; Non-Metastatic Cells 4 Protein Expressed In; NM23H4; nm23-H4; NME 4; NME-4; anti-NME4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2G1
• Purity/Purification: NME4 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 187

• Applicable Applications for anti-NME4 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; IHC: 1:50; WB: 1:2000
• Biological Significance: The nucleoside diphosphate (NDP) kinases are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates.
• Cross-Reactivity: Human
• Immunogen: NME4 antibody was raised in mouse using a full length recombinant protein of human NME4 (NP_005000) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NME4 protein (red) or empty vector (blue) stained using NME4 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NME4 protein (Right) or empty vector (Left) detected with NME4 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NME4 protein in paraffin embedded Human liver tissue using NME4 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NME4 protein using NME4 antibody)

Related Product Information for anti-NME4 antibody

Mouse monoclonal NME4 antibody

Product Categories/Family for anti-NME4 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4826862
• NCBI GeneID: 4833
• NCBI Accession #: NP_005000
• NCBI GenBank Nucleotide #: NM_005009.2
• UniProt Accession #: O00746; Q5U0M9; A2IDD0
• Molecular Weight: 12,979 Da
• NCBI Official Full Name: nucleoside diphosphate kinase, mitochondrial isoform a
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 4
• NCBI Official Symbol: NME4
• NCBI Official Synonym Symbols: NDPK-D; NM23H4; nm23-H4
• NCBI Protein Information: nucleoside diphosphate kinase, mitochondrial
• UniProt Protein Name: Nucleoside diphosphate kinase, mitochondrial
• UniProt Gene Name: NME4
• UniProt Synonym Gene Names: NM23D; NDK; NDP kinase, mitochondrial; NDPKD
• UniProt Entry Name: NDKM_HUMAN

NCBI Description

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]

Uniprot Description

NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 2.7.4.6; Mitochondrial; Kinase, other; Kinase, nucleoside diphosphate; Other group; NDK family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding; metal ion binding; protein complex binding; nucleoside diphosphate kinase activity; ATP binding

Biological Process: regulation of apoptosis; GTP biosynthetic process; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside metabolic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process