FN3K sirna

FN3K siRNA (Mouse)

Cat. Num. AAA8235344

• Gene Names: Fn3k; 2310074G21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FN3K; FN3K siRNA (Mouse); Fructosamine-3-kinase; FN3K sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FN3K siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 309

• Applicable Applications for FN3K sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FN3K gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FN3K sirna

siRNA to inhibit FN3K expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11528496
• NCBI GeneID: 63828
• NCBI Accession #: NP_071297.1
• NCBI GenBank Nucleotide #: NM_022014.4
• UniProt Accession #: Q9ER35; Q9D6N6
• Molecular Weight: 35,032 Da
• NCBI Official Full Name: fructosamine-3-kinase isoform a
• NCBI Official Synonym Full Names: fructosamine 3 kinase
• NCBI Official Symbol: Fn3k
• NCBI Official Synonym Symbols: 2310074G21Rik
• NCBI Protein Information: fructosamine-3-kinase
• UniProt Protein Name: Fructosamine-3-kinase
• Protein Family: Fructosamine-3-kinase
• UniProt Gene Name: Fn3k
• UniProt Entry Name: FN3K_MOUSE

Uniprot Description

FN3K: May initiate a process leading to the deglycation of fructoselysine and of glycated proteins. May play a role in the phosphorylation of 1-deoxy-1-morpholinofructose (DMF), fructoselysine, fructoseglycine, fructose and glycated lysozyme. Belongs to the fructosamine kinase family.

Protein type: Kinase, other; EC 2.7.1.-

Cellular Component: cytosol

Molecular Function: phenol kinase activity; phosphatidylinositol phosphate kinase activity; cobinamide kinase activity; cyclin-dependent protein kinase activating kinase regulator activity; ATP-dependent polynucleotide kinase activity; inositol tetrakisphosphate 2-kinase activity; (S)-lactate 2-kinase activity; glycerate 2-kinase activity; LPPG:FO 2-phospho-L-lactate transferase activity; cyclin-dependent protein kinase regulator activity; phosphorylase kinase regulator activity; GTP-dependent polynucleotide kinase activity; phosphoinositide 3-kinase activity; transferase activity; heptose 7-phosphate kinase activity; transferase activity, transferring phosphorus-containing groups; phytol kinase activity; kinase activity; eukaryotic elongation factor-2 kinase regulator activity; fructosamine-3-kinase activity; cytidine kinase activity; phosphofructokinase activity; aminoglycoside phosphotransferase activity; polynucleotide kinase activity; eukaryotic elongation factor-2 kinase activator activity

Biological Process: fructosamine metabolic process; phosphorylation