SDS-PAGE
(Coomassie blue staining of human cell lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, MCF7; 3, HeLa; 4, HT-29; 5, 293; 6, Hep G2; 7, A-549; 8, SK-N-SH; and 9, PC-3.)
Cell Line I Blot Immunoblot
Human Cell Line I Blot
Applications
Western Blot
Synonyms
Cell Line I Blot; Human Cell Line I Blot; Cell Line I Blot immunoblot
Applicable Applications for Cell Line I Blot immunoblot
Western Blot (WB)
Application Notes
For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
Species
Human
Quantity Loaded Per Lane
15 ug
Samples Loaded
Lanes: 1, protein markers; 2, MCF7; 3, HeLa; 4, HT-29; 5, 293; 6, Hep G2; 7, A-549; 8, SK-N-SH; and 9, PC-3.
Note
Lysate lane order may vary; please consult accompanying shipment paperwork.
Preparation and Storage
Store at 4 degree C. The blot can be reused after stripping.
SDS-PAGE
(Coomassie blue staining of human cell lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, MCF7; 3, HeLa; 4, HT-29; 5, 293; 6, Hep G2; 7, A-549; 8, SK-N-SH; and 9, PC-3.)
SDS-PAGE
(Coomassie blue staining of human cell lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, MCF7; 3, HeLa; 4, HT-29; 5, 293; 6, Hep G2; 7, A-549; 8, SK-N-SH; and 9, PC-3.)
Related Product Information for Cell Line I Blot immunoblot
The human cell line blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human cell lines with 15 ug of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The cell lines included in the blot are MCF7 (breast; adenocarcinoma), HeLa (cervix; adenocarcinoma), HT-29 (colon; adenocarcinoma), 293 (kidney; transformed with adenovirus 5 DNA), Hep G2 (liver; hepatocellular carcinoma), A-549 (lung; carcinoma), SK-N-SH (brain; neuroblastoma), and PC-3 (prostate;adenocarcinoma).
Similar Products
Product Notes
The Cell Line I Blot (Catalog #AAA153748) is an Immunoblot and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cell Line I Blot can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature. Researchers should empirically determine the suitability of the Cell Line I Blot for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cell Line I Blot, Immunoblot" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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