FN1 sirna

FN1 siRNA (Mouse)

Cat. Num. AAA8223202

• Gene Names: Fn1; Fn; Fn-1; E330027I09
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FN1; FN1 siRNA (Mouse); Fibronectin; FN; FN1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FN1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2477

• Applicable Applications for FN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FN1 sirna

siRNA to inhibit FN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46849812
• NCBI GeneID: 14268
• NCBI Accession #: NP_034363.1
• NCBI GenBank Nucleotide #: NM_010233.2
• UniProt Accession #: P11276; Q61567; Q61568; Q61569; Q64233; Q80UI4; G5E8B8
• Molecular Weight: 272,538 Da
• NCBI Official Full Name: fibronectin isoform a
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: Fn; Fn-1; E330027I09
• NCBI Protein Information: fibronectin
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_MOUSE

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrinogen complex; apical plasma membrane; ER-Golgi intermediate compartment; extracellular region; basement membrane; basal lamina

Molecular Function: heparin binding; integrin binding; protein binding; protease activator activity; protease binding; mercury ion binding

Biological Process: integrin activation; extracellular matrix organization and biogenesis; wound healing; positive regulation of chemotaxis; cell-matrix adhesion; positive regulation of axon extension; glial cell migration; regulation of cell shape; cell activation; acute-phase response; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; angiogenesis; peptide cross-linking; cell adhesion; negative regulation of apoptosis; positive regulation of cell migration