FLI1 sirna

FLI1 siRNA (Mouse)

Cat. Num. AAA8236334

• Gene Names: Fli1; Sic1; EWSR2; Fli-1; SIC-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FLI1; FLI1 siRNA (Mouse); FLI-1; Friend leukemia integration 1 transcription factor; Retroviral integration site protein Fli-1; FLI1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FLI1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 452

• Applicable Applications for FLI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FLI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FLI1 sirna

siRNA to inhibit FLI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679807
• NCBI GeneID: 14247
• NCBI Accession #: NP_032052.1
• NCBI GenBank Nucleotide #: NM_008026.5
• UniProt Accession #: P26323
• Molecular Weight: 51,002 Da
• NCBI Official Full Name: Friend leukemia integration 1 transcription factor
• NCBI Official Synonym Full Names: Friend leukemia integration 1
• NCBI Official Symbol: Fli1
• NCBI Official Synonym Symbols: Sic1; EWSR2; Fli-1; SIC-1
• NCBI Protein Information: Friend leukemia integration 1 transcription factor
• UniProt Protein Name: Friend leukemia integration 1 transcription factor
• Protein Family: Friend leukemia integration 1 transcription factor
• UniProt Gene Name: Fli1
• UniProt Synonym Gene Names: Fli-1
• UniProt Entry Name: FLI1_MOUSE

Uniprot Description

FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; organ morphogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cellular process