FANCD2 sirna

FANCD2 siRNA (Mouse)

Cat. Num. AAA8206070

• Gene Names: Fancd2; FA4; FAD; FACD; FA-D2; FANCD; AU015151; BB137857; 2410150O07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FANCD2; FANCD2 siRNA (Mouse); Fanconi anemia group D2 protein homolog; Protein FACD2; FANCD2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FANCD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 129

• Applicable Applications for FANCD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FANCD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FANCD2 sirna

siRNA to inhibit FANCD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 282721083
• NCBI GeneID: 211651
• NCBI Accession #: NP_001028416.2
• NCBI GenBank Nucleotide #: NM_001033244.3
• UniProt Accession #: Q80V62; Q9CWC7
• Molecular Weight: 14,683 Da
• NCBI Official Full Name: Fanconi anemia group D2 protein homolog
• NCBI Official Synonym Full Names: Fanconi anemia, complementation group D2
• NCBI Official Symbol: Fancd2
• NCBI Official Synonym Symbols: FA4; FAD; FACD; FA-D2; FANCD; AU015151; BB137857; 2410150O07Rik
• NCBI Protein Information: Fanconi anemia group D2 protein homolog
• UniProt Protein Name: Fanconi anemia group D2 protein homolog
• Protein Family: FANCD2 opposite strand protein
• UniProt Gene Name: Fancd2
• UniProt Synonym Gene Names: Protein FACD2
• UniProt Entry Name: FACD2_MOUSE

Uniprot Description

FANCD2: Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching. Interacts directly with FANCE and FANCI. Interacts with USP1 and MEN1. The ubiquitinated form specifically interacts with BRCA1 and BLM. Both the nonubiquitinated and the monoubiquitinated forms interact with BRCA2; this interaction is mediated by phosphorylated FANCG and the complex also includes XCCR3. The ubiquitinated form specifically interacts with MTMR15/FAN1 (via UBZ-type zinc finger), leading to recruit MTMR15/FAN1 to sites of DNA damage. Interacts with DCLRE1B/Apollo. Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas. Highly expressed in testis, where expression is restricted to maturing spermatocytes. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; condensed chromosome; nucleus

Molecular Function: protein binding

Biological Process: multicellular organismal development; gamete generation; synapsis; response to gamma radiation; cell cycle; DNA repair; response to DNA damage stimulus