FAM20C sirna

FAM20C siRNA (Mouse)

Cat. Num. AAA8213435

• Gene Names: Fam20c; DMP4; DMP-4; C76981; GEF-CK; mKIAA4081
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FAM20C; FAM20C siRNA (Mouse); DMP4; KIAA4081; Extracellular serine/threonine protein kinase FAM20C; Dentin matrix protein 4; DMP-4; Golgi-enriched fraction casein kinase; GEF-CK; FAM20C sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FAM20C siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 579

• Applicable Applications for FAM20C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FAM20C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FAM20C sirna

siRNA to inhibit FAM20C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 167234439
• NCBI GeneID: 80752
• NCBI Accession #: NP_085042.2
• NCBI GenBank Nucleotide #: NM_030565.6
• UniProt Accession #: Q5MJS3; Q4FJP0; Q571A3; Q6PKA8; Q8JZP7
• Molecular Weight: 65,766 Da
• NCBI Official Full Name: extracellular serine/threonine protein kinase FAM20C
• NCBI Official Synonym Full Names: family with sequence similarity 20, member C
• NCBI Official Symbol: Fam20c
• NCBI Official Synonym Symbols: DMP4; DMP-4; C76981; GEF-CK; mKIAA4081
• NCBI Protein Information: extracellular serine/threonine protein kinase FAM20C
• UniProt Protein Name: Extracellular serine/threonine protein kinase FAM20C
• Protein Family: Extracellular serine/threonine protein kinase
• UniProt Gene Name: Fam20c
• UniProt Synonym Gene Names: GEF-CK
• UniProt Entry Name: FA20C_MOUSE

Uniprot Description

FAM20C: an atypical serine protein kinase that localizes within the Golgi apparatus and is secreted. Distantly related to the Drosophila Golgi-localized kinase Four-jointed. Phosphorylates secretory proteins including the caseins and members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) protein family, which modulate biomineralization. Colocalizes with the Golgi resident protein GM130. Preferentially phosphorylates its targets within the S-x-E/pS motif. Requires Mn2+ as an alternative to Mg2+ as the activating cation. Defects in FAM20C are the cause of Raine syndrome (RNS). RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Secreted, signal peptide

Cellular Component: Golgi apparatus; extracellular space; extracellular region

Molecular Function: transferase activity; protein serine/threonine kinase activity; metal ion binding; calcium ion binding; kinase activity

Biological Process: positive regulation of osteoblast differentiation; biomineral formation; regulation of fibroblast growth factor receptor signaling pathway; positive regulation of bone mineralization; protein amino acid phosphorylation; regulation of phosphorus metabolic process; phosphorylation; skeletal development