Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Intercellular adhesion molecule 4 (ICAM4) Standard Curve.)
Human Intercellular Adhesion Molecule 4 (ICAM4) ELISA Kit | ICAM4 elisa kit
Human Intercellular Adhesion Molecule 4 (ICAM4) ELISA Kit
Gene Names
ICAM4; LW; CD242
Reactivity
Human
Synonyms
Intercellular Adhesion Molecule 4 (ICAM4); Human Intercellular Adhesion Molecule 4 (ICAM4) ELISA Kit; CD242; LW; CD242 antigen; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; intercellular adhesion molecule 4; intercellular adhesion molecule 4 (LW blood group); ICAM4 elisa kit
Reactivity
Human
Specificity
Human Intercellular adhesion molecule 4 (ICAM4) ELISA Kit has high sensitivity and excellent specificity for detection of Human ICAM4. No significant cross-reactivity or interference between Human ICAM4 and analogues was observed.
Sequence Length
271
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Intercellular adhesion molecule 4 (ICAM4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Intercellular adhesion molecule 4 (ICAM4) Standard Curve.)
Related Product Information for ICAM4 elisa kit
Description: This Human Intercellular adhesion molecule 4 (ICAM4) ELISA Kit employs a two-site sandwich ELISA to quantitate ICAM4 in samples. An antibody specific for ICAM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ICAM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ICAM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ICAM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The LW glycoprotein has recently been renamed ICAM-4 due to its similarity to intercellular adhesion molecule, although exactly which integrins bind to ICAM-4 is subject to controversy. The function of ICAM-4 is not fully understood but appears to be restricted to erythroid cells. During in vitro erythropoesis, LW appears at either the erythroid colony forming stage or later at the proerythroblast stage. A vital part of erythropoesis is the clustering of erythroblasts around bone marrow macrophages to form erythroblastic islands. The erythroblast is then able to remove its nucleus, which is in turn ingested and broken down by the macrophages, to become a mature erythrocyte. During this process ICAM-4 binds to VLA-4, an erythroblast binding site, on adjacent erythroblasts and to alpha integrins on macrophages to help stabilise the erythroblastic islands.
Background: The LW glycoprotein has recently been renamed ICAM-4 due to its similarity to intercellular adhesion molecule, although exactly which integrins bind to ICAM-4 is subject to controversy. The function of ICAM-4 is not fully understood but appears to be restricted to erythroid cells. During in vitro erythropoesis, LW appears at either the erythroid colony forming stage or later at the proerythroblast stage. A vital part of erythropoesis is the clustering of erythroblasts around bone marrow macrophages to form erythroblastic islands. The erythroblast is then able to remove its nucleus, which is in turn ingested and broken down by the macrophages, to become a mature erythrocyte. During this process ICAM-4 binds to VLA-4, an erythroblast binding site, on adjacent erythroblasts and to alpha integrins on macrophages to help stabilise the erythroblastic islands.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
intercellular adhesion molecule 4 isoform 1
NCBI Official Synonym Full Names
intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
NCBI Official Symbol
ICAM4
NCBI Official Synonym Symbols
LW; CD242
NCBI Protein Information
intercellular adhesion molecule 4
UniProt Protein Name
Intercellular adhesion molecule 4
Protein Family
UniProt Gene Name
ICAM4
UniProt Synonym Gene Names
LW; ICAM-4; LW blood group protein
NCBI Description
This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. This ICAM protein contains 2 Ig-like C2-type domains and binds to the leukocyte adhesion LFA-1 protein. The molecular basis of the LW(A)/LW(B) blood group antigens is a single aa variation at position 100; Gln-100=LW(A) and Arg-100=LW(B). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.
Research Articles on ICAM4
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Product Notes
The Human ICAM4 icam4 (Catalog #AAA9716045) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716045 ELISA Kit recognizes Human ICAM4. It is sometimes possible for the material contained within the vial of "Intercellular Adhesion Molecule 4 (ICAM4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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