F11R sirna

F11R siRNA (Mouse)

Cat. Num. AAA8237393

• Gene Names: F11r; JAM; Jcam; JAM-1; JAM-A; Jcam1; Ly106; ESTM33; AA638916; 9130004G24
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: F11R; F11R siRNA (Mouse); JAM1; JCAM; JCAM1; Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; CD antigen CD321; F11R sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: F11R siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 300

• Applicable Applications for F11R sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse F11R gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for F11R sirna

siRNA to inhibit F11R expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 27734847
• NCBI GeneID: 16456
• NCBI Accession #: NP_766235.1
• NCBI GenBank Nucleotide #: NM_172647.2
• UniProt Accession #: O88792; Q8VC39
• Molecular Weight: 32,424 Da
• NCBI Official Full Name: junctional adhesion molecule A
• NCBI Official Synonym Full Names: F11 receptor
• NCBI Official Symbol: F11r
• NCBI Official Synonym Symbols: JAM; Jcam; JAM-1; JAM-A; Jcam1; Ly106; ESTM33; AA638916; 9130004G24
• NCBI Protein Information: junctional adhesion molecule A
• UniProt Protein Name: Junctional adhesion molecule A
• Protein Family: Junctional adhesion molecule
• UniProt Gene Name: F11r
• UniProt Synonym Gene Names: Jam1; Jcam; Jcam1; JAM-A; JAM-1
• UniProt Entry Name: JAM1_MOUSE

Uniprot Description

JAM-A: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. Belongs to the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Cell adhesion

Cellular Component: microtubule cytoskeleton; tight junction; membrane; plasma membrane; integral to membrane; intercellular junction; cytoplasmic vesicle; cell junction

Molecular Function: protein binding; PDZ domain binding

Biological Process: viral reproduction; epithelial cell differentiation; actomyosin structure organization and biogenesis; cell adhesion; positive regulation of blood pressure; intestinal absorption