ERN2 sirna

ERN2 siRNA (Mouse)

Cat. Num. AAA8225442

• Gene Names: Ern2; Ern1; Ire1; Ire1b
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERN2; ERN2 siRNA (Mouse); IRE2; Serine/threonine-protein kinase/endoribonuclease IRE2; Endoplasmic reticulum-to-nucleus signaling 2; Inositol-requiring protein 2; Ire1-beta; IRE1b; mIre1; ERN2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERN2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 911

• Applicable Applications for ERN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERN2 sirna

siRNA to inhibit ERN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124248583
• NCBI GeneID: 26918
• NCBI Accession #: NP_036146.2
• NCBI GenBank Nucleotide #: NM_012016.2
• UniProt Accession #: Q9Z2E3; Q3U5E3
• Molecular Weight: 101,262 Da
• NCBI Official Full Name: serine/threonine-protein kinase/endoribonuclease IRE2
• NCBI Official Synonym Full Names: endoplasmic reticulum (ER) to nucleus signalling 2
• NCBI Official Symbol: Ern2
• NCBI Official Synonym Symbols: Ern1; Ire1; Ire1b
• NCBI Protein Information: serine/threonine-protein kinase/endoribonuclease IRE2
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE2
• Protein Family: Serine/threonine-protein kinase/endoribonuclease
• UniProt Gene Name: Ern2
• UniProt Synonym Gene Names: IRE1b; mIre1
• UniProt Entry Name: ERN2_MOUSE

Uniprot Description

IRE2: Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: Ribonuclease; Protein kinase, Other; Kinase, protein; Membrane protein, integral; Apoptosis; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; IRE family

Cellular Component: membrane; endoplasmic reticulum; integral to membrane; intracellular

Molecular Function: hydrolase activity; metal ion binding; nucleotide binding; magnesium ion binding; protein kinase activity; ribonuclease activity; transferase activity; protein serine/threonine kinase activity; endonuclease activity; bidentate ribonuclease III activity; transferase activity, transferring phosphorus-containing groups; kinase activity; catalytic activity; ATP binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; metabolic process; apoptosis; rRNA catabolic process; cell cycle arrest; apoptotic chromosome condensation; negative regulation of transcription, DNA-dependent; mRNA processing; protein amino acid phosphorylation; phosphorylation; response to unfolded protein