ERCC8 sirna

ERCC8 siRNA (Mouse)

Cat. Num. AAA8225924

• Gene Names: Ercc8; Csa; Ckn1; 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC8; ERCC8 siRNA (Mouse); CKN1; CSA; DNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA homolog; ERCC8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERCC8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 397

• Applicable Applications for ERCC8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERCC8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC8 sirna

siRNA to inhibit ERCC8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110625789
• NCBI GeneID: 71991
• NCBI Accession #: NP_082318.2
• NCBI GenBank Nucleotide #: NM_028042.3
• UniProt Accession #: Q8CFD5; Q3U066; Q8VDC4; Q9CWK7
• Molecular Weight: 43,689 Da
• NCBI Official Full Name: DNA excision repair protein ERCC-8
• NCBI Official Synonym Full Names: excision repaiross-complementing rodent repair deficiency, complementation group 8
• NCBI Official Symbol: Ercc8
• NCBI Official Synonym Symbols: Csa; Ckn1; 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik
• NCBI Protein Information: DNA excision repair protein ERCC-8
• UniProt Protein Name: DNA excision repair protein ERCC-8
• Protein Family: DNA excision repair protein
• UniProt Gene Name: Ercc8
• UniProt Synonym Gene Names: Ckn1; Csa
• UniProt Entry Name: ERCC8_MOUSE

Uniprot Description

ERCC8: Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription- coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Defects in ERCC8 are the cause of Cockayne syndrome type A (CSA). Cockayne syndrome is a rare disorder characterized by cutaneous sensitivity to sunlight, abnormal and slow growth, cachectic dwarfism, progeroid appearance, progressive pigmentary retinopathy and sensorineural deafness. There is delayed neural development and severe progressive neurologic degeneration resulting in mental retardation. Two clinical forms are recognized: in the classical form or Cockayne syndrome type 1, the symptoms are progressive and typically become apparent within the first few years or life; the less common Cockayne syndrome type 2 is characterized by more severe symptoms that manifest prenatally. Cockayne syndrome shows some overlap with certain forms of xeroderma pigmentosum. Unlike xeroderma pigmentosum, patients with Cockayne syndrome do not manifest increased freckling and other pigmentation abnormalities in the skin and have no significant increase in skin cancer. Defects in ERCC8 are the cause of UV-sensitive syndrome type 2 (UVSS2). An autosomal recessive disorder characterized by cutaneous photosensitivity and mild freckling in the absence of neurological abnormalities or skin tumors. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; DNA repair, damage; Ubiquitin conjugating system

Cellular Component: nucleotide-excision repair complex; nuclear matrix; protein complex; nucleus

Molecular Function: protein complex binding; ubiquitin-protein ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; positive regulation of DNA repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; response to oxidative stress; DNA repair; response to DNA damage stimulus; response to X-ray; response to UV