anti-Human, Mouse MAFF Antibody | anti-MAFF antibody

MAFF Antibody

Cat. Num. AAA855401

• Gene Names: MAFF; U-MAF; hMafF
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: MAFF; MAFF Antibody; Transcription factor MafF; V-maf musculoaponeurotic fibrosarcoma oncogene homolog F; U-Maf; anti-MAFF antibody

• Reactivity: Human, Mouse
• Specificity: MAFF antibody detects endogenous levels of total MAFF protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 164

• Applicable Applications for anti-MAFF antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human MAFF.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MAFF antibody.)

Product Categories/Family for anti-MAFF antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Ota T., Nat. Genet. 36:40-45(2004). Moran J.A., Biochem. J. 361:371-377(2002). Dunham I., Nature 402:489-495(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 239048350
• NCBI GeneID: 23764
• NCBI Accession #: NP_001155044.1
• NCBI GenBank Nucleotide #: NM_001161572.1
• UniProt Accession #: Q9ULX9; Q9Y525; B4DV49
• Molecular Weight: 14,569 Da
• NCBI Official Full Name: transcription factor MafF isoform a
• NCBI Official Synonym Full Names: MAF bZIP transcription factor F
• NCBI Official Symbol: MAFF
• NCBI Official Synonym Symbols: U-MAF; hMafF
• NCBI Protein Information: transcription factor MafF
• UniProt Protein Name: Transcription factor MafF
• Protein Family: MaFF-interacting protein
• UniProt Gene Name: MAFF

NCBI Description

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:8932385). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2L1/NRF1, and recruiting them to specific DNA-binding sites. Interacts with the upstream promoter region of the oxytocin receptor gene (PubMed:8932385, PubMed:16549056). May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition (PubMed:10527846).