ERBB2 sirna

ERBB2 siRNA (Mouse)

Cat. Num. AAA8207782

• Gene Names: Erbb2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; mKIAA3023
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERBB2; ERBB2 siRNA (Mouse); KIAA3023; NEU; Receptor tyrosine-protein kinase erbB-2; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; p185erbB2; CD antigen CD340; ERBB2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERBB2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,256

• Applicable Applications for ERBB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERBB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERBB2 sirna

siRNA to inhibit ERBB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 54873610
• NCBI GeneID: 13866
• NCBI Accession #: NP_001003817.1
• NCBI GenBank Nucleotide #: NM_001003817.1
• UniProt Accession #: P70424; Q61525; Q6ZPE0
• Molecular Weight: 138,579 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2
• NCBI Official Synonym Full Names: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• NCBI Official Symbol: Erbb2
• NCBI Official Synonym Symbols: Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; mKIAA3023
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb2
• UniProt Synonym Gene Names: Kiaa3023; Neu
• UniProt Entry Name: ERBB2_MOUSE

Uniprot Description

HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Oncoprotein; Kinase, protein; Membrane protein, integral; Protein kinase, TK; TK group; EGFR family

Cellular Component: microvillus; basolateral plasma membrane; lateral loop; integral to membrane; lipid raft; postsynaptic membrane; membrane; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex

Molecular Function: protein C-terminus binding; identical protein binding; nucleotide binding; Hsp90 protein binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; protein kinase activity; transferase activity; protein binding; transmembrane receptor activity; protein heterodimerization activity; ubiquitin protein ligase binding; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; glycoprotein binding; ATP binding

Biological Process: myelination; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; peptidyl-tyrosine phosphorylation; wound healing; positive regulation of translation; protein amino acid autophosphorylation; heart development; motor axon guidance; signal transduction; protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; sympathetic nervous system development; cell surface receptor linked signal transduction; glial cell differentiation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; response to axon injury; neuromuscular junction development; negative regulation of immature T cell proliferation in the thymus; regulation of microtubule-based process; nervous system development; transcription, DNA-dependent; positive regulation of cell growth; peripheral nervous system development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of transcription from RNA polymerase III promoter; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of GTPase activity