EPHA8 sirna

EPHA8 siRNA (Mouse)

Cat. Num. AAA8207267

• Gene Names: Epha8; Eek; Hek3; AW047546; mKIAA1459
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPHA8; EPHA8 siRNA (Mouse); EEK; Ephrin type-A receptor 8; EPH- and ELK-related kinase; Tyrosine-protein kinase receptor EEK; EPHA8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EPHA8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,004

• Applicable Applications for EPHA8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EPHA8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPHA8 sirna

siRNA to inhibit EPHA8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 154240697
• NCBI GeneID: 13842
• NCBI Accession #: NP_031965.2
• NCBI GenBank Nucleotide #: NM_007939.2
• UniProt Accession #: O09127; A3KG07
• Molecular Weight: 110,705 Da
• NCBI Official Full Name: ephrin type-A receptor 8
• NCBI Official Synonym Full Names: Eph receptor A8
• NCBI Official Symbol: Epha8
• NCBI Official Synonym Symbols: Eek; Hek3; AW047546; mKIAA1459
• NCBI Protein Information: ephrin type-A receptor 8
• UniProt Protein Name: Ephrin type-A receptor 8
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha8
• UniProt Synonym Gene Names: Eek
• UniProt Entry Name: EPHA8_MOUSE

Uniprot Description

EphA8: a receptor tyrosine kinase of the Eph family. A receptor for members of the ephrin-A family: ephrin A2, A3 and A5. Plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Contains 1 sterile alpha motif (SAM) domain and 2 fibronectin type III domains.

Protein type: Membrane protein, integral; EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Eph family

Cellular Component: neuron projection; cell projection; membrane; integral to plasma membrane; plasma membrane; integral to membrane; endosome

Molecular Function: transferase activity; protein binding; GPI-linked ephrin receptor activity; protein-tyrosine kinase activity; ephrin receptor activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: substrate-bound cell migration; regulation of cell adhesion; nervous system development; axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; multicellular organismal development; positive regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; regulation of cell adhesion mediated by integrin; positive regulation of MAPKKK cascade; ephrin receptor signaling pathway; cell adhesion; phosphorylation; neurite development; transmembrane receptor protein tyrosine kinase signaling pathway; neuron remodeling