EIF4H sirna

EIF4H siRNA (Mouse)

Cat. Num. AAA8210780

• Gene Names: Eif4h; Ef4h; Wscr1; Wbscr1; eIF-4H; AU018978; D5Ertd355e; E430026L18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF4H; EIF4H siRNA (Mouse); WBSCR1; Eukaryotic translation initiation factor 4H; eIF-4H; Williams-Beuren syndrome chromosomal region 1 protein homolog; EIF4H sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EIF4H siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 248

• Applicable Applications for EIF4H sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EIF4H gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF4H sirna

siRNA to inhibit EIF4H expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15808988
• NCBI GeneID: 22384
• NCBI Accession #: NP_291039.1
• NCBI GenBank Nucleotide #: NM_033561.2
• UniProt Accession #: Q9WUK2; Q9WUK3
• Molecular Weight: 25,186 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4H isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4H
• NCBI Official Symbol: Eif4h
• NCBI Official Synonym Symbols: Ef4h; Wscr1; Wbscr1; eIF-4H; AU018978; D5Ertd355e; E430026L18Rik
• NCBI Protein Information: eukaryotic translation initiation factor 4H
• UniProt Protein Name: Eukaryotic translation initiation factor 4H
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: Eif4h
• UniProt Synonym Gene Names: Wbscr1; eIF-4H
• UniProt Entry Name: IF4H_MOUSE

NCBI Description

This gene encodes eukaryotic translation initiation factor 4H (eIF4H) that plays a critical role in the process of protein synthesis. The encoded protein is an RNA-binding protein that, in concert with other translation initiation factors, helps unwind the 5' cap-proximal region of mRNA to prepare it for ribosomal attachment. Mice lacking the encoded protein displayed growth retardation with a significant reduction of body weight, a smaller brain volume and altered brain morphology. Behaviorally, mice lacking the encoded protein exhibit severe impairments of fear-related associative learning and memory formation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Uniprot Description

EIF4H: eukaryotic translation initiation factor 4H stimulates protein translation. May stimulate the activities of EIF4A, EIF4B and EIF4F during the steps involving mRNA binding and utilization in the initiation of protein biosynthesis. Binds mRNA. Two alternatively spliced isoforms have been described. The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.

Protein type: Translation initiation; RNA-binding; Translation

Cellular Component: membrane; cytoplasm

Molecular Function: nucleic acid binding; RNA binding; translation initiation factor activity; nucleotide binding

Biological Process: developmental growth; translation; sexual reproduction; translational initiation