EIF4G2 sirna

EIF4G2 siRNA (Mouse)

Cat. Num. AAA8208809

• Gene Names: Eif4g2; p97; Nat1; DAP-5; Natm1; AA589388; E130105L11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF4G2; EIF4G2 siRNA (Mouse); NAT1; Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Novel APOBEC-1 target 1; Translation repressor NAT1; p97; EIF4G2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EIF4G2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 868

• Applicable Applications for EIF4G2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EIF4G2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF4G2 sirna

siRNA to inhibit EIF4G2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110630015
• NCBI GeneID: 13690
• NCBI Accession #: NP_001035221.1
• NCBI GenBank Nucleotide #: NM_001040131.2
• UniProt Accession #: Q62448; P97867; Q0VGZ3; Q3UNC1; Q5XKD8; Q6P1Z0; Q921U3
• Molecular Weight: 97,894 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4 gamma 2 isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4, gamma 2
• NCBI Official Symbol: Eif4g2
• NCBI Official Synonym Symbols: p97; Nat1; DAP-5; Natm1; AA589388; E130105L11Rik
• NCBI Protein Information: eukaryotic translation initiation factor 4 gamma 2
• UniProt Protein Name: Eukaryotic translation initiation factor 4 gamma 2
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: Eif4g2
• UniProt Synonym Gene Names: eIF-4-gamma 2; eIF-4G 2; eIF4G 2
• UniProt Entry Name: IF4G2_MOUSE

NCBI Description

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G, that contains the binding sites for eIF4A and eIF3; eIF4G in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA, which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EIF4G2: a general repressor of translation by forming translationally inactive complexes. Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Interacts with the protein kinases Mnk1 and -2. Binds EIF4A and EIF3. Cleaved by some caspases and viral proteases.

Protein type: RNA-binding; Translation initiation; Translation

Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytoplasm

Molecular Function: protein binding; translation factor activity, nucleic acid binding; RNA binding; translation initiation factor activity

Biological Process: regulation of translation; translation; translational initiation; regulation of translational initiation