EHMT2 sirna

EHMT2 siRNA (Mouse)

Cat. Num. AAA8232747

• Gene Names: Ehmt2; G9a; Bat8; NG36; KMT1C; D17Ertd710e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EHMT2; EHMT2 siRNA (Mouse); BAT8; G9A; NG36; Histone-lysine N-methyltransferase EHMT2; Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Protein G9a; EHMT2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EHMT2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1229

• Applicable Applications for EHMT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EHMT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EHMT2 sirna

siRNA to inhibit EHMT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 557440749
• NCBI GeneID: 110147
• NCBI Accession #: NP_001273502.1
• NCBI GenBank Nucleotide #: NM_001286573.1
• UniProt Accession #: Q9Z148; Q6PE08; Q8K4R6; Q8K4R7; Q9Z149; A2CG75
• Molecular Weight: 131,772 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase EHMT2 isoform c
• NCBI Official Synonym Full Names: euchromatic histone lysine N-methyltransferase 2
• NCBI Official Symbol: Ehmt2
• NCBI Official Synonym Symbols: G9a; Bat8; NG36; KMT1C; D17Ertd710e
• NCBI Protein Information: histone-lysine N-methyltransferase EHMT2
• UniProt Protein Name: Histone-lysine N-methyltransferase EHMT2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Ehmt2
• UniProt Synonym Gene Names: Bat8; G9a; Ng36; H3-K9-HMTase 3
• UniProt Entry Name: EHMT2_MOUSE

Uniprot Description

EHMT2: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleolus; chromosome; nucleus

Molecular Function: methyltransferase activity; monomethylamine methyltransferase activity; trimethylamine methyltransferase activity; RNA methyltransferase activity; tRNA (cytosine)-methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; histone-lysine N-methyltransferase activity; tRNA (guanine) methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific); rRNA (adenine-N6,N6-)-dimethyltransferase activity; selenocysteine methyltransferase activity; arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; protein-arginine N-methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; hydroxyneurosporene-O-methyltransferase activity; transferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; histone lysine N-methyltransferase activity (H3-K9 specific); C-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; zinc ion binding; metal ion binding; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (adenine)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; p53 binding; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; protein binding; mRNA methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity

Biological Process: methylation; synaptonemal complex assembly; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; germ cell development; peptidyl-lysine methylation; regulation of transcription from RNA polymerase II promoter; histone methylation; organ growth; cytosine methylation within a CG sequence; fertilization; long-term memory; peptidyl-lysine di-methylation; DNA methylation; regulation of histone H3-K4 methylation; regulation of histone H3-K9 methylation; spermatid development; regulation of DNA replication; histone H3-K9 methylation