EGFBP2 sirna

EGFBP2 siRNA (Mouse)

Cat. Num. AAA8234098

• Gene Names: Egfbp2; mGk-13; Egfbp-2; Klk1b26
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EGFBP2; EGFBP2 siRNA (Mouse); EGFBP-2; KLK-13; KLK13; Epidermal growth factor-binding protein type B; EGF-BP B; Glandular kallikrein K13; mGK-13; Prorenin-converting enzyme 1; PRECE-1; Tissue kallikrein 13; EGFBP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EGFBP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 261

• Applicable Applications for EGFBP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EGFBP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EGFBP2 sirna

siRNA to inhibit EGFBP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 402244288
• NCBI GeneID: 13647
• NCBI Accession #: NP_034245.3
• NCBI GenBank Nucleotide #: NM_010115.6
• UniProt Accession #: P36368; P00754
• Molecular Weight: 28,689 Da
• NCBI Official Full Name: epidermal growth factor-binding protein type B preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor binding protein type B
• NCBI Official Symbol: Egfbp2
• NCBI Official Synonym Symbols: mGk-13; Egfbp-2; Klk1b26
• NCBI Protein Information: epidermal growth factor-binding protein type B
• UniProt Protein Name: Epidermal growth factor-binding protein type B
• Protein Family: Epidermal growth factor-binding protein
• UniProt Gene Name: Egfbp2
• UniProt Synonym Gene Names: Egfbp-2; Klk-13; Klk13; EGF-BP B; mGK-13; PRECE-1
• UniProt Entry Name: EGFB2_MOUSE

NCBI Description

The protein encoded by this gene belongs to the kallikrein family, which is a highly homologous group of serine proteases encoded by a cluster of related genes on chromosome 7. This gene has been shown to function as both a prorenin converting enzyme and as epidermal growth factor (EGF)-binding protein involved in the maturation of EGF (PMIDs: 1918045, 3322387, 9685728). This gene is thought to be distinct from Klk1b26 gene (GeneID:16618), with which it shares 98% identity (PMID:1959648, 9685728), however, it is not clear if both genes are present in all strains of mice. Blast analyses suggest that this gene is missing from the current reference (GRCm38, based on C57BL/6J genome) and alternate Celera (based on mixed strain genomes) assemblies. Therefore, the RefSeq for this locus was based on sequence from the ICR strain, which has been reported in literature (PMID:1959648) to contain both genes. [provided by RefSeq, Aug 2012]

Uniprot Description

Egfbp2: Cleaves REN2 at a dibasic site to yield mature renin. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.119

Cellular Component: extracellular space

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: zymogen activation; proteolysis