Rabbit anti-Human VAP1 Polyclonal Antibody | anti-VAP1 antibody

VAP1, ID (Membrane Primary Amine Oxidase, Copper Amine Oxidase, Semicarbazide-sensitive Amine Oxidase, SSAO, Vascular Adhesion Protein 1, VAP-1, HPAO, AOC3) (PE)

Cat. Num. AAA6505497

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VAP1; Polyclonal Antibody; ID (Membrane Primary Amine Oxidase; Copper Amine Oxidase; Semicarbazide-sensitive Amine Oxidase; SSAO; Vascular Adhesion Protein 1; VAP-1; HPAO; AOC3) (PE); anti-VAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VAP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-VAP1 antibody: Western Blot (WB), FLISA, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 613-640 in the central region of human VAP1, UniProt Accession #NP_003725.1; Q16853.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS627415. VAP1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human colon carcinoma usingMBS627415, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS627415 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of CEM cells using MBS627415 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-VAP1 antibody

Antibodies; Enzymes; Oxidase

References

1. Lalor, P.F., et.al., J. Immunol. 169(2), 983-992 (2002). 2. Salmi, M., et.al., Am. J. Pathol. 161(6), 2255-2262 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4502119
• NCBI GeneID: 8639
• NCBI Accession #: NP_003725.1
• NCBI GenBank Nucleotide #: NM_003734.3
• UniProt Accession #: Q16853; Q45F94; B2RCI5; K7ESB3; L0L8N9
• NCBI Official Full Name: membrane primary amine oxidase isoform 1
• NCBI Official Synonym Full Names: amine oxidase, copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase; copper amine oxidase; placenta copper monamine oxidase; semicarbazide-sensitive amine oxidase; amine oxidase, copper containing 3 (vascular adhesion protein 1)
• UniProt Protein Name: Membrane primary amine oxidase
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1
• UniProt Entry Name: AOC3_HUMAN

NCBI Description

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Cell adhesion; Oxidoreductase; Amino Acid Metabolism - phenylalanine; Other Amino Acids Metabolism - beta-alanine; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine; EC 1.4.3.21

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cell surface; microvillus; cytoplasm; plasma membrane; integral to membrane

Molecular Function: amine oxidase activity; protein binding; protein homodimerization activity; copper ion binding; protein heterodimerization activity; cation channel activity; calcium ion binding; quinone binding

Biological Process: response to antibiotic; amine metabolic process; cell adhesion; inflammatory response; cation transport