EDC4 sirna

EDC4 siRNA (Mouse)

Cat. Num. AAA829056

• Gene Names: Edc4; BC022641
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EDC4; EDC4 siRNA (Mouse); Enhancer of mRNA-decapping protein 4; EDC4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EDC4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1406

• Applicable Applications for EDC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EDC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EDC4 sirna

siRNA to inhibit EDC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 666875868
• NCBI GeneID: 234699
• NCBI Accession #: NP_001288029.1
• NCBI GenBank Nucleotide #: NM_001301100.1
• UniProt Accession #: Q3UJB9; Q3U6U8; Q7TS78; Q8R223
• Molecular Weight: 150,653 Da
• NCBI Official Full Name: enhancer of mRNA-decapping protein 4 isoform 1
• NCBI Official Synonym Full Names: enhancer of mRNA decapping 4
• NCBI Official Symbol: Edc4
• NCBI Official Synonym Symbols: BC022641
• NCBI Protein Information: enhancer of mRNA-decapping protein 4
• UniProt Protein Name: Enhancer of mRNA-decapping protein 4
• Protein Family: Enhancer of mRNA-decapping protein
• UniProt Gene Name: Edc4
• UniProt Entry Name: EDC4_MOUSE

NCBI Description

The protein encoded by this gene is thought to promote mRNA decay, and is known to interact with several mRNA decapping proteins. In humans, decreased expression of this gene prevents the accumulation of mRNA decapping proteins to mRNA processing bodies (P-body). Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

EDC4: a protein required for the 5' a?? 3' pathway of mRNA decapping and degradation-based post-transcriptional gene silencing. The proteins involved in 5' a?? 3' mRNA decay include DCP2, DCP1A, EDC3, EDC4 and DDX6, and are concentrated in cytoplasmic structures called mRNA "processing bodies" (P-bodies). Shortening of the 3'-poly(A) tail and removal of PABP precedes cleavage of the 5'-mRNA cap by a complex containing decapping enzymes 1A and 2 (DCP1A/DCP2). EDC4 enhances the catalytic activity of DCP2 in vitro. Shown to be required for silencing by miRNAs in Drosophila melanogaster. Two alternatively spliced human isoforms have been described.

Protein type: RNA processing

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; membrane; cytoplasm; nucleus

Molecular Function: protein binding