DYNLT1A sirna

DYNLT1A siRNA (Mouse)

Cat. Num. AAA8203005

• Gene Names: Dynlt1f; Dynlt1e; 100040631
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DYNLT1A; DYNLT1A siRNA (Mouse); TCTEL1; TCTEX-1; TCTEX1; Dynein light chain Tctex-type 1; Activator of G-protein signaling 2; AGS2; T-complex testis-specific protein 1; DYNLT1A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DYNLT1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 113

• Applicable Applications for DYNLT1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DYNLT1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DYNLT1A sirna

siRNA to inhibit DYNLT1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 262231811
• NCBI GeneID: 100040531
• NCBI Accession #: NP_001160099.1
• NCBI GenBank Nucleotide #: NM_001166627.1
• UniProt Accession #: P51807; Q5M8S6
• Molecular Weight: 12,483 Da
• NCBI Official Full Name: dynein light chain Tctex-type 1F isoform 1
• NCBI Official Synonym Full Names: dynein light chain Tctex-type 1F
• NCBI Official Symbol: Dynlt1f
• NCBI Official Synonym Symbols: Dynlt1e; 100040631
• NCBI Protein Information: dynein light chain Tctex-type 1F
• UniProt Protein Name: Dynein light chain Tctex-type 1
• UniProt Gene Name: Dynlt1
• UniProt Synonym Gene Names: Tctel1; Tctex-1; Tctex1; AGS2
• UniProt Entry Name: DYLT1_MOUSE

Uniprot Description

DYNLT1: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein. Homodimer (Probable). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non- catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with DYNC1I1 and DYNC1I2. Interacts with RHO. Interacts with DOC2A, DOC2B and SCN10A. Interacts with PVR. Interacts with SVIL isoform 2. Interacts with BMPR2. Interacts with GNB1; the interaction occurs in presence of guanine nucleotide-binding protein G(T) subunit gamma; the interaction diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in presence of guanine nucleotide-binding protein G(T) subunit gamma. Interacts with human papillomavirus 16 L2 protein; this interaction is essential for virus intracellular transport during entry. Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells. Belongs to the dynein light chain Tctex-type family.

Protein type: Microtubule-binding; Motility/polarity/chemotaxis; Motor

Cellular Component: Golgi apparatus; axonemal dynein complex; cytoplasmic dynein complex

Molecular Function: identical protein binding

Biological Process: microtubule-based process