DRD3 sirna

DRD3 siRNA (Mouse)

Cat. Num. AAA8223632

• Gene Names: Drd3; D3R
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DRD3; DRD3 siRNA (Mouse); D(3) dopamine receptor; Dopamine D3 receptor; DRD3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DRD3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 446

• Applicable Applications for DRD3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DRD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DRD3 sirna

siRNA to inhibit DRD3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6681221
• NCBI GeneID: 13490
• NCBI Accession #: NP_031903.1
• NCBI GenBank Nucleotide #: NM_007877.1
• UniProt Accession #: P30728
• Molecular Weight: 47,168 Da
• NCBI Official Full Name: D(3) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D3
• NCBI Official Symbol: Drd3
• NCBI Official Synonym Symbols: D3R
• NCBI Protein Information: D(3) dopamine receptor
• UniProt Protein Name: D(3) dopamine receptor
• Protein Family: D(3) dopamine receptor
• UniProt Gene Name: Drd3
• UniProt Entry Name: DRD3_MOUSE

Uniprot Description

DRD3: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1). ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Cellular Component: cell projection; membrane; integral to plasma membrane; endocytic vesicle; apical part of cell; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; dopamine D2 receptor-like receptor activity; protein domain specific binding; protein binding; signal transducer activity; dopamine receptor activity; dopamine binding; drug binding; D1 dopamine receptor binding

Biological Process: synaptic transmission, dopaminergic; regulation of lipid metabolic process; prepulse inhibition; musculoskeletal movement, spinal reflex action; response to morphine; locomotory behavior; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of dopamine secretion; dopamine receptor, adenylate cyclase inhibiting pathway; synaptic transmission; behavioral response to cocaine; positive regulation of cell proliferation; renin-angiotensin regulation of blood volume; visual learning; negative regulation of blood pressure; circadian regulation of gene expression; negative regulation of sodium:hydrogen antiporter activity; dopamine receptor, adenylate cyclase activating pathway; positive regulation of cytokinesis; response to drug; positive regulation of dopamine receptor signaling pathway; negative regulation of protein secretion; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; regulation of cAMP metabolic process; negative regulation of adenylate cyclase activity; response to amphetamine; regulation of multicellular organism growth; response to cocaine; arachidonic acid secretion; regulation of circadian sleep/wake cycle, sleep; regulation of locomotion; cellular calcium ion homeostasis; negative regulation of oligodendrocyte differentiation; G-protein coupled receptor protein signaling pathway; response to ethanol; acid secretion; negative regulation of dopamine receptor signaling pathway; G-protein coupled receptor internalization; dopamine receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter