Rabbit anti-Mouse, Human BRD3 Polyclonal Antibody | anti-BRD3 antibody

Anti-BRD3 Antibody (aa672-702) IHC-plus

Cat. Num. AAA249865

• Gene Names: BRD3; ORFX; RING3L
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Ammonium sulfate precipitation
• Synonyms: BRD3; Polyclonal Antibody; Anti-BRD3 Antibody (aa672-702) IHC-plus; Rabbit Polyclonal to Human BRD3; Human BRD3; Bromodomain-containing 3; Fsrg2; KIAA0043; ORFX; RING3-like protein; RING3L; Bromodomain containing 3; anti-BRD3 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Specificity: This BRD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672-702 amino acids from the C-terminal region of human BRD3.
• Purity/Purification: Ammonium sulfate precipitation
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 726

• Applicable Applications for anti-BRD3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (1:100); WB (1:1000)
• Immunogen Species: BRD3 antibody was raised against human.
• Epitope: aa672-702
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months.; For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-BRD3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(The anti-BRD3 antibody is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).)

Related Product Information for anti-BRD3 antibody

BRD3 Antibody, Bromodomain-containing 3 Antibody, Fsrg2 Antibody, KIAA0043 Antibody, ORFX Antibody, RING3-like protein Antibody, RING3L Antibody, Bromodomain containing 3 Antibody Description: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.

NCBI and Uniprot Product Information

• NCBI GI #: 11067749
• NCBI GeneID: 8019
• NCBI Accession #: NP_031397.1
• NCBI GenBank Nucleotide #: NM_007371.3
• UniProt Accession #: Q15059; Q4G5Y3; Q5T1R7; Q8N5M3; Q92645; B1APD9
• NCBI Official Full Name: bromodomain-containing protein 3
• NCBI Official Synonym Full Names: bromodomain containing 3
• NCBI Official Symbol: BRD3
• NCBI Official Synonym Symbols: ORFX; RING3L
• NCBI Protein Information: bromodomain-containing protein 3
• UniProt Protein Name: Bromodomain-containing protein 3
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD3
• UniProt Synonym Gene Names: KIAA0043; RING3L
• UniProt Entry Name: BRD3_HUMAN

NCBI Description

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleus

Molecular Function: chromatin binding; protein binding

Biological Process: chromatin modification; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent