DPY19L2 sirna

DPY19L2 siRNA (Mouse)

Cat. Num. AAA8226896

• Gene Names: Dpy19l2; Gm18376; 4932443J21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DPY19L2; DPY19L2 siRNA (Mouse); Probable C-mannosyltransferase DPY19L2; Dpy-19-like protein 2; Protein dpy-19 homolog 2; DPY19L2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DPY19L2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 773

• Applicable Applications for DPY19L2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DPY19L2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DPY19L2 sirna

siRNA to inhibit DPY19L2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 261245007
• NCBI GeneID: 320752
• NCBI Accession #: NP_001159679.1
• NCBI GenBank Nucleotide #: NM_001166207.1
• UniProt Accession #: P0CW70
• Molecular Weight: 89,882 Da
• NCBI Official Full Name: probable C-mannosyltransferase DPY19L2
• NCBI Official Synonym Full Names: dpy-19-like 2 (C. elegans)
• NCBI Official Symbol: Dpy19l2
• NCBI Official Synonym Symbols: Gm18376; 4932443J21Rik
• NCBI Protein Information: probable C-mannosyltransferase DPY19L2
• UniProt Protein Name: Probable C-mannosyltransferase DPY19L2
• Protein Family: Probable C-mannosyltransferase
• UniProt Gene Name: Dpy19l2
• UniProt Entry Name: D19L2_MOUSE

Uniprot Description

DPY19L2: Required during spermatogenesis for sperm head elongation and acrosome formation. Defects in DPY19L2 are the cause of spermatogenic failure type 9 (SPGF9). An infertility disorder caused by spermatogenesis defects. The most prominent feature is the malformation of the acrosome, which can be totally absent in most severe cases. Additional features are an abnormal nuclear shape and abnormal arrangement of the mitochondria of the spermatozoon. Deletions in DPY19L2 are probably the major cause of SPGF9. Belongs to the dpy-19 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; integral to membrane; nuclear inner membrane; nucleus

Molecular Function: alpha(1,6)-fucosyltransferase activity; oligosaccharyl transferase activity; acetylgalactosaminyltransferase activity; alpha(1,3)-fucosyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; glycolipid mannosyltransferase activity; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; fucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; transferase activity; 9-phenanthrol glycosyltransferase activity; beta-1,4-mannosyltransferase activity; cellulose synthase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity

Biological Process: multicellular organismal development; spermatogenesis; protein amino acid C-linked glycosylation via 2'-alpha-mannosyl-L-tryptophan; cell differentiation; spermatid development