DNMT1 sirna

DNMT1 siRNA (Mouse)

Cat. Num. AAA8214961

• Gene Names: Dnmt1; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DNMT1; DNMT1 siRNA (Mouse); DNMT; MET1; UIM; DNA; cytosine-5)-methyltransferase 1; Dnmt1; Met-1; DNA methyltransferase MmuI; DNA MTase MmuI; M.MmuI; MCMT; DNMT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DNMT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1620

• Applicable Applications for DNMT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNMT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DNMT1 sirna

siRNA to inhibit DNMT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 327180732
• NCBI GeneID: 13433
• NCBI Accession #: NP_001186360.2
• NCBI GenBank Nucleotide #: NM_001199431.1
• UniProt Accession #: P13864; P97413; Q80ZU3; Q9CSC6; Q9QXX6
• Molecular Weight: 169,996 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: DNA methyltransferase (cytosine-5) 1
• NCBI Official Symbol: Dnmt1
• NCBI Official Synonym Symbols: Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: Dnmt1
• UniProt Synonym Gene Names: Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI
• UniProt Entry Name: DNMT1_MOUSE

Uniprot Description

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: EC 2.1.1.37; Transcription regulation; Methyltransferase, DNA; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase

Cellular Component: centric heterochromatin; heterochromatin; protein complex; cell soma; cytoplasm; replication fork; nucleus

Molecular Function: protein domain specific binding; methyltransferase activity; methyl-CpG binding; unmethylated CpG binding; zinc ion binding; RNA binding; metal ion binding; histone deacetylase binding; transcription factor binding; DNA-methyltransferase activity; transferase activity; DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; double-stranded DNA binding; estrogen receptor binding; chromatin binding; catalytic activity

Biological Process: negative regulation of histone H3-K9 methylation; methylation; maintenance of DNA methylation; transcription, DNA-dependent; metabolic process; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; gene silencing; chromatin modification; regulation of cell proliferation; cytosine methylation within a CG sequence; regulation of transcription, DNA-dependent; regulation of gene expression; DNA methylation; S-adenosylmethioninamine metabolic process; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine